4933432B09Rik Activators
Chemical activators of Fam227a, including caffeine, forskolin, PMA, resveratrol, A23187, thapsigargin, ionomycin, calyculin A, cantharidin, okadaic acid, bryostatin, and TPA, modulate the activation of Fam227a through diverse cellular mechanisms. Caffeine and forskolin act as direct activators by elevating intracellular cyclic AMP (cAMP) levels, initiating the activation of protein kinase A (PKA). PKA can then phosphorylate Fam227a, directly activating its functionality. PMA and bryostatin activate Fam227a by stimulating protein kinase C (PKC), which can phosphorylate and activate Fam227a directly or through downstream signaling pathways. Additionally, resveratrol inhibits SIRT1, a negative regulator of Fam227a, promoting its acetylation and activation.
Agents like A23187, ionomycin, and thapsigargin induce calcium influx, leading to the activation of various signaling pathways involving protein kinases. These kinases can directly phosphorylate Fam227a or modulate its activity through intermediary proteins, contributing to functional activation. Calyculin A, cantharidin, and okadaic acid indirectly activate Fam227a by inhibiting protein phosphatases, particularly PP1 and PP2A. Inhibition of these phosphatases results in sustained phosphorylation and activation of Fam227a and its downstream targets. Overall, this comprehensive set of chemical activators acts through distinct but interconnected cellular pathways to influence Fam227a activation, providing valuable insights into potential strategies for modulating its function within a cellular context.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $32.00 $66.00 $95.00 $188.00 $760.00 | 13 | |
Caffeine activates Fam227a by antagonizing adenosine receptors, particularly A2A receptors, which leads to increased cAMP levels. Elevated cAMP levels activate protein kinase A (PKA), which can phosphorylate and activate Fam227a directly or through downstream signaling pathways. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates Fam227a by stimulating protein kinase C (PKC), which can phosphorylate and activate Fam227a directly or through downstream signaling pathways. PKC activation leads to various cellular responses, including gene expression changes and protein activation. | ||||||
Resveratrol | 501-36-0 | sc-200808 sc-200808A sc-200808B | 100 mg 500 mg 5 g | $60.00 $185.00 $365.00 | 64 | |
Resveratrol activates Fam227a by inhibiting SIRT1, a negative regulator of Fam227a activity. By inhibiting SIRT1, resveratrol prevents the deacetylation of Fam227a, leading to its activation and subsequent functions in the cell. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 activates Fam227a by inducing calcium influx into the cell. Increased intracellular calcium levels can activate various signaling pathways, including those involving protein kinases, which may directly phosphorylate and activate Fam227a or modulate its activity through other proteins. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin activates Fam227a indirectly by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump, leading to calcium release from the endoplasmic reticulum. Elevated intracellular calcium levels can activate various signaling pathways that may indirectly activate Fam227a. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin activates Fam227a by facilitating calcium influx into the cell. Increased intracellular calcium levels can activate various signaling pathways, including those involving protein kinases, which may directly phosphorylate and activate Fam227a or modulate its activity through other proteins. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Calyculin A activates Fam227a by inhibiting protein phosphatases, particularly protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Inhibition of these phosphatases leads to sustained phosphorylation and activation of Fam227a and its downstream targets. | ||||||
Cantharidin | 56-25-7 | sc-201321 sc-201321A | 25 mg 100 mg | $81.00 $260.00 | 6 | |
Cantharidin activates Fam227a indirectly by inhibiting protein phosphatases, particularly protein phosphatase 2A (PP2A). Inhibition of PP2A leads to sustained phosphorylation and activation of Fam227a and its downstream targets. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid activates Fam227a indirectly by inhibiting protein phosphatases, particularly protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Inhibition of these phosphatases leads to sustained phosphorylation and activation of Fam227a and its downstream targets. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $240.00 | 9 | |
Bryostatin activates Fam227a by activating protein kinase C (PKC), which can phosphorylate and activate Fam227a directly or through downstream signaling pathways. PKC activation leads to various cellular responses, including gene expression changes and protein activation. | ||||||