Topo IIβ Antibody (B-6): sc-365952

Topo IIβ Antibody (B-6) is a mouse monoclonal IgG1 antibody that detects Topo IIβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Topo IIβ is a crucial nuclear enzyme that plays a significant role in maintaining DNA topology during critical cellular processes, including DNA replication and transcription. Unlike Topo IIα, which is more widely expressed, Topo IIβ exhibits a unique expression pattern particularly important in neuronal development and function. The distinct C-terminal region of Topo IIβ suggests interactions with specific proteins involved in neuronal signaling pathways, thereby influencing cellular responses to DNA damage and stress. Understanding the specific interactions and functions of Topo IIβ is vital, as dysregulation has been implicated in various cancers and neurological disorders. With an ability to facilitate transient breaking and rejoining of DNA strands, Topo IIβ serves as a potential target for therapeutic interventions, making anti-Topo IIβ antibody (B-6) an essential tool for researchers studying molecular mechanisms underlying these diseases.

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Topo IIβ Antibody (B-6) References:

1. Murine transgenic cells lacking DNA topoisomerase IIbeta are resistant to acridines and mitoxantrone: analysis of cytotoxicity and cleavable complex formation.  |  Errington, F., et al. 1999. Mol Pharmacol. 56: 1309-16. PMID: 10570059
2. Enhanced etoposide sensitivity following adenovirus-mediated human topoisomerase IIalpha gene transfer is independent of topoisomerase IIbeta.  |  Zhou, Z., et al. 2001. Br J Cancer. 85: 747-51. PMID: 11531262
3. A breaking strategy for topoisomerase IIbeta/PARP-1-dependent regulated transcription.  |  Ju, BG. and Rosenfeld, MG. 2006. Cell Cycle. 5: 2557-60. PMID: 17106262
4. Regulation of DNA Topoisomerase IIbeta through RNA-dependent association with heterogeneous nuclear ribonucleoprotein U (hnRNP U).  |  Kawano, S., et al. 2010. J Biol Chem. 285: 26451-60. PMID: 20554522
5. Tyrosine 656 in topoisomerase IIβ is important for the catalytic activity of the enzyme: Identification based on artifactual +80-Da modification at this site.  |  Grozav, AG., et al. 2011. Proteomics. 11: 829-42. PMID: 21280220
6. Nuclear dynamics of topoisomerase IIβ reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain.  |  Onoda, A., et al. 2014. Nucleic Acids Res. 42: 9005-20. PMID: 25034690
7. Immunohistochemical investigation of topoIIβ, H3K27me3 and JMJD3 expressions in medulloblastoma.  |  Chen, J., et al. 2017. Pathol Res Pract. 213: 975-981. PMID: 28554742
8. Effects of hsa-miR-9-3p and hsa-miR-9-5p on Topoisomerase IIβ Expression in Human Leukemia K562 Cells with Acquired Resistance to Etoposide.  |  Carvajal-Moreno, J., et al. 2023. J Pharmacol Exp Ther. 384: 265-276. PMID: 36410793
9. Silymarin protects against doxorubicin induced cardiotoxicity by down-regulating topoisomerase IIβ expression in mice.  |  İpek, E. and Tunca, R. 2023. Biotech Histochem. 98: 412-423. PMID: 37272191
10. Decreased expression of topoisomerase IIbeta in poly(ADP-ribose) polymerase-deficient cells.  |  Canitrot, Y., et al. 1998. Nucleic Acids Res. 26: 5134-8. PMID: 9801310

The preferred Topo IIβ antibody is Topo IIβ Antibody (H-8): sc-25330.