Topo IIβ Antibody (A-12): sc-365071
- Topo IIβ Antibody (A-12) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1579-1611 near the C-terminus of Topo IIβ of human origin
- Anti-Topo II beta Antibody (A-12) is recommended for detection of Topo IIβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Topo II beta Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Topo IIβ Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with Topo IIβ Antibody (A-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Topo IIβ Antibody (A-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Topo IIβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Topo IIβ antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Topo IIβ (A-12) monoclonal antibody recognizes a protein that plays a crucial role in maintaining DNA integrity during critical cellular processes such as replication and transcription by introducing transient double-strand breaks in DNA, allowing for relaxation of supercoiled DNA structures. This function prevents DNA tangling and ensures proper chromosome segregation during cell division, which is essential for genomic stability. Differential expression of Topo IIβ, particularly in specific tissues, suggests unique regulatory roles in cellular processes compared to Topo IIα isoform. Understanding distinct functions of Topo IIβ can provide insights into potential therapeutic targets in cancer treatment, where modulation of topoisomerase activity is a common strategy for disrupting cancer cell proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Topo IIβ Antibody (A-12) References:
- DNA topoisomerase IIbeta and neural development. | Yang, X., et al. 2000. Science. 287: 131-4. PMID: 10615047
- Involvement of DNA topoisomerase IIbeta in neuronal differentiation. | Tsutsui, K., et al. 2001. J Biol Chem. 276: 5769-78. PMID: 11106659
- Topoisomerase IIbeta activates a subset of neuronal genes that are repressed in AT-rich genomic environment. | Sano, K., et al. 2008. PLoS One. 3: e4103. PMID: 19116664
- Novel ametantrone-amsacrine related hybrids as topoisomerase IIβ poisons and cytotoxic agents. | Zagotto, G., et al. 2014. Arch Pharm (Weinheim). 347: 728-37. PMID: 25042690
- DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression. | Zaim, M. and Isik, S. 2018. Stem Cell Res Ther. 9: 114. PMID: 29695291
- Mutations in topoisomerase IIβ result in a B cell immunodeficiency. | Broderick, L., et al. 2019. Nat Commun. 10: 3644. PMID: 31409799
- The Role of Topoisomerase IIβ in the Mechanisms of Action of the Doxorubicin Cardioprotective Agent Dexrazoxane. | Hasinoff, BB., et al. 2020. Cardiovasc Toxicol. 20: 312-320. PMID: 31773441
- Down regulation of DNA topoisomerase IIβ exerts neurodegeneration like effect through Rho GTPases in cellular model of Parkinson's disease by Down regulating tyrosine hydroxylase. | Yeman, KB. and Isik, S. 2021. Neurol Res. 43: 464-473. PMID: 33402057
- Genome-wide prediction of topoisomerase IIβ binding by architectural factors and chromatin accessibility. | Martínez-García, PM., et al. 2021. PLoS Comput Biol. 17: e1007814. PMID: 33465072
- Structure-Activity Relationship Study of Dexrazoxane Analogues Reveals ICRF-193 as the Most Potent Bisdioxopiperazine against Anthracycline Toxicity to Cardiomyocytes Due to Its Strong Topoisomerase IIβ Interactions. | Jirkovská, A., et al. 2021. J Med Chem. 64: 3997-4019. PMID: 33750129
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo IIβ Antibody (A-12) | sc-365071 | 200 µg/ml | $322.00 | |||
Topo IIβ Antibody (A-12): m-IgGκ BP-HRP Bundle | sc-522130 | 200 µg Ab, 40 µg BP | $361.00 | |||
Topo IIβ Antibody (A-12) AC | sc-365071 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Topo IIβ Antibody (A-12) HRP | sc-365071 HRP | 200 µg/ml | $322.00 | |||
Topo IIβ Antibody (A-12) FITC | sc-365071 FITC | 200 µg/ml | $336.00 | |||
Topo IIβ Antibody (A-12) PE | sc-365071 PE | 200 µg/ml | $349.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 488 | sc-365071 AF488 | 200 µg/ml | $364.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 546 | sc-365071 AF546 | 200 µg/ml | $364.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 594 | sc-365071 AF594 | 200 µg/ml | $364.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 647 | sc-365071 AF647 | 200 µg/ml | $364.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 680 | sc-365071 AF680 | 200 µg/ml | $364.00 | |||
Topo IIβ Antibody (A-12) Alexa Fluor® 790 | sc-365071 AF790 | 200 µg/ml | $364.00 | |||
Topo IIβ (A-12) Neutralizing Peptide | sc-365071 P | 100 µg/0.5 ml | $69.00 |