Topo II beta Antibody (A-12) recommended for detection of Topo IIβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Topo IIβ Antibody (A-12): sc-365071

Topo IIβ Antibody (A-12) is rated 4.2 out of 5 by 5.
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Datasheets

    • Topo IIβ Antibody (A-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1579-1611 near the C-terminus of Topo IIβ of human origin
    • Anti-Topo II beta Antibody (A-12) is recommended for detection of Topo IIβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Topo II beta Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Topo IIβ (A-12): sc-365071.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Topo IIβ Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with Topo IIβ Antibody (A-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Is this antibody available as ChIP grade?

Asked by: Amit5629489
Thank you for your question. We should be able to provide this product as a TransCruz reagent for Gel Supershift and ChIP applications. Please get in touch with our technical service so we can make the necessary arrangements.
Answered by: Tech Support Europe
Date published: 2020-06-30

What application is the blocking peptide sc-365071 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from A good result for Topo IIβ WB Gastric cancer cells BGC823 were uesd to confirm the expression of Topo IIβ. Transfering time was 360 minutes in 250mA. Blocking in milk for hour. Incubateing with first antibody(1:500) overnight. Incubateing with second antibody(1:2000) for one hour
Date published: 2017-11-18
Rated 2 out of 5 by from TOPOIIB A-12 in Western Blot I received this as a free sample antibody and tested it out at a 1:200 dilution using Whole Cell Extracts from four different cell lines (HeLa, HEK293, HEK293T, and Mouse Embryonic Fibroblast cells). The expected size for TOPO11B is 180 kDa and I do see something at the correct height in maybe the last 3 lanes (the HeLa could just be loaded with less extract). The exposure of the blots was weird for this antibody and another Topoisomerase antibody I also tested (Santa Cruz H-8).
Date published: 2017-10-20
Rated 5 out of 5 by from versatile and consistent! This monoclonal Topo II beta Antibody (A-12) is a versatile reagent with strong in-house data in Western blot, IF and IHC(P).
Date published: 2017-01-31
Rated 5 out of 5 by from Great immunofluorescence nuclear staining Great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by from Nice Western Blot data of Topo II expression in U Nice Western Blot data of Topo II expression in U-937, MEG-01, CCRF-CEM and K-562 nuclear extracts. -SCBT QC
Date published: 2013-02-23
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