Topo II Antibody (T22C5): sc-65743
- Topo II Antibody (T22C5) is a mouse monoclonal IgG1 κ Topo II antibody, cited in 4 publications, provided at 200 µg/ml
- raised against topoisomerase II purified from 6-18 hour embryos of Drosophila melanogaster origin
- recommended for detection of topoisomerase II of d origin by WB and IP
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Topo II Antibody (T22C5) for WB applications. These reagents are now offered in bundles with Topo II Antibody (T22C5) (see ordering information below).
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Topo II Antibody (T22C5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Topo II protein of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-Topo II antibody (T22C5) is available as a non-conjugated format. DNA topoisomerase II (Topo II) plays a crucial role in managing DNA topology during replication, transcription, and recombination. Topo II (T22C5) monoclonal antibody recognizes a 1447 amino acid protein encoded by the Top2 gene in Drosophila, exhibiting structural similarities to Topo II found in other eukaryotic organisms. Anti-Topo II antibody (T22C5) targets a protein featuring an N-terminal region homologous to bacterial type II topoisomerase B subunit, a central region resembling DNA gyrase A subunit, and a C-terminal region characterized by alternating positively and negatively charged amino acids, important for DNA interaction. Due to Topo II′s pivotal role in DNA metabolism and status as a target for various anticancer therapies, Topo II (T22C5) antibody serves as a valuable tool in both basic and clinical research.
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Topo II Antibody (T22C5) References:
- Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively. | Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
- Structure of the human type I DNA topoisomerase gene. | Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
- Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells. | Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
- Structure of the Drosophila DNA topoisomerase II gene. Nucleotide sequence and homology among topoisomerases II. | Wyckoff, E., et al. 1989. J Mol Biol. 205: 1-13. PMID: 2538621
- Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. | Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
- cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment. | D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
- The mechanisms of DNA topoisomerases. | Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
- A model for the mechanism of human topoisomerase I. | Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo II Antibody (T22C5) | sc-65743 | 200 µg/ml | $322.00 | |||
Topo II Antibody (T22C5): m-IgG Fc BP-HRP Bundle | sc-539193 | 200 µg Ab; 10 µg BP | $361.00 | |||
Topo II Antibody (T22C5): m-IgG1 BP-HRP Bundle | sc-541317 | 200 µg Ab; 20 µg BP | $361.00 |