Topo II Antibody (T21D4): sc-65742

Topo II Antibody (T21D4) is a mouse monoclonal IgG2b antibody that detects Topo II in Drosophila by western blotting (WB). Topo II is a crucial nuclear enzyme that plays a significant role in maintaining the topological structure of DNA within eukaryotic cells by transiently breaking and rejoining DNA strands. This process is vital for various cellular functions, including DNA replication, recombination, and transcription, making Topo II an important target for numerous anticancer therapies. Topo II consists of 1,447 amino acids and features distinct structural domains: an N-terminal region homologous to the B subunit of bacterial type II topoisomerase, a central region resembling the A subunit of DNA gyrase, and a C-terminal region characterized by alternating positively and negatively charged amino acids. These structural features are essential for enzymatic activity, allowing Topo II to introduce transient double-strand breaks in DNA, which is critical for the resolution of DNA tangles and supercoils that arise during cellular processes. Anti-Topo II antibody (T21D4) is an invaluable tool for researchers studying the dynamics of DNA topology and the mechanisms of action of topoisomerase inhibitors in cancer treatment.

The preferred Topo IIα antibody is Topo IIα Antibody (F-12): sc-365916.