Topo I Antibody (H-5): sc-271285
- Topo I Antibody (H-5) is a mouse monoclonal IgG1 κ, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 685-765 of Topo I of human origin
- Anti-Topo I Antibody (H-5) is recommended for detection of Topo I of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-Topo I Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Topo I Antibody (H-5) for WB applications. This reagent is now offered in a bundle with Topo I Antibody (H-5) (see ordering information below).
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Topo I Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Topoisomerase I (Topo I) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Topo I (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Topo I plays a crucial role in DNA metabolism by introducing transient breaks in one strand of the DNA helix, which is essential for alleviating torsional strain during processes such as DNA replication and transcription. This function allows proper DNA unwinding, facilitating access of replication and transcription machinery to genetic material. Dysregulation of Topo I activity can lead to genomic instability and has been implicated in various cancers, making Topo I a significant target for therapeutic intervention. Additionally, Topo I interacts with several specific proteins, including transcription factors and other DNA-binding proteins, further underscoring Topo I′s importance in maintaining cellular homeostasis and regulating gene expression. Anti-Topo I antibody (H-5)′s ability to effectively detect this vital protein across multiple species and applications makes anti-Topo I antibody (H-5) an invaluable tool for researchers studying DNA dynamics and related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Topo I Antibody (H-5) References:
- Effects of mutations in the F361 to R364 region of topoisomerase I (Topo I), in the presence and absence of 9-aminocamptothecin, on the Topo I-DNA interaction. | Pond, CD., et al. 1999. Anticancer Drugs. 10: 647-53. PMID: 10507314
- WRN protects against topo I but not topo II inhibitors by preventing DNA break formation. | Christmann, M., et al. 2008. DNA Repair (Amst). 7: 1999-2009. PMID: 18805512
- Overexpression of Lewis(y) antigen protects ovarian cancer RMG-1 cells from carboplatin-induced apoptosis by the upregulation of Topo-I and Topo-II β. | Wang, C., et al. 2011. Anat Rec (Hoboken). 294: 961-9. PMID: 21542140
- CES2, ABCG2, TS and Topo-I primary and synchronous metastasis expression and clinical outcome in metastatic colorectal cancer patients treated with first-line FOLFIRI regimen. | Silvestris, N., et al. 2014. Int J Mol Sci. 15: 15767-77. PMID: 25198900
- Novel 2'-alkoxymethyl substituted klavuzon derivatives as inhibitors of Topo I and CRM1. | Çetinkaya, H., et al. 2020. Bioorg Chem. 103: 104162. PMID: 32890988
- The Mode of SN38 Derivatives Interacting with Nicked DNA Mimics Biological Targeting of Topo I Poisons. | Bocian, W., et al. 2021. Int J Mol Sci. 22: PMID: 34299090
- Synthesis of indolo/pyrroloazepinone-oxindoles as potential cytotoxic, DNA-intercalating and Topo I inhibitors. | Kadagathur, M., et al. 2022. Bioorg Chem. 122: 105706. PMID: 35240414
- N-2-(Phenylamino) Benzamide Derivatives as Dual Inhibitors of COX-2 and Topo I Deter Gastrointestinal Cancers via Targeting Inflammation and Tumor Progression. | Li, J., et al. 2022. J Med Chem. 65: 10481-10505. PMID: 35868003
- Potential Multifunctional Bioactive Compounds from Dysosma versipellis Explored by Bioaffinity Ultrafiltration-HPLC/MS with Topo I, Topo II, COX-2 and ACE2. | Feng, H., et al. 2022. J Inflamm Res. 15: 4677-4692. PMID: 35996684
- Insight on the Interaction between the Camptothecin Derivative and DNA Oligomer Mimicking the Target of Topo I Inhibitors. | Bocian, W., et al. 2022. Molecules. 27: PMID: 36296539
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo I Antibody (H-5) | sc-271285 | 200 µg/ml | $316.00 | |||
Topo I Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-525794 | 200 µg Ab; 10 µg BP | $354.00 | |||
Topo I Antibody (H-5) AC | sc-271285 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Topo I Antibody (H-5) HRP | sc-271285 HRP | 200 µg/ml | $316.00 | |||
Topo I Antibody (H-5) FITC | sc-271285 FITC | 200 µg/ml | $330.00 | |||
Topo I Antibody (H-5) PE | sc-271285 PE | 200 µg/ml | $343.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 488 | sc-271285 AF488 | 200 µg/ml | $357.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 546 | sc-271285 AF546 | 200 µg/ml | $357.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 594 | sc-271285 AF594 | 200 µg/ml | $357.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 647 | sc-271285 AF647 | 200 µg/ml | $357.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 680 | sc-271285 AF680 | 200 µg/ml | $357.00 | |||
Topo I Antibody (H-5) Alexa Fluor® 790 | sc-271285 AF790 | 200 µg/ml | $357.00 |