Shank 1 Antibody (E-1): sc-398630

Shank 1 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Shank 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Shank 1 (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Shank 1 protein plays a crucial role in the postsynaptic density (PSD), a specialized structure essential for synaptic signaling and plasticity. Located beneath the postsynaptic membrane of excitatory synapses, PSD serves as a molecular hub that organizes and anchors glutamate receptors, thereby facilitating effective neurotransmission. Shank proteins, including Shank 1, contain PDZ domains vital for mediating protein-protein interactions, allowing recruitment of various signaling molecules and receptors to the synapse. This recruitment is critical for maintaining synaptic integrity and function, ensuring necessary components for synaptic signaling are properly localized. Additionally, Shank 1 interaction with other proteins, such as betaPIX and PAK, is essential for regulating dendritic spine structure at synaptic transmission sites. Shank 1′s ability to influence receptor complex assembly and signaling pathways underscores Shank 1′s importance in both normal brain development and neurodevelopmental disorder pathophysiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Shank 1 Antibody (E-1) References:

1. Characterization of the Shank family of synaptic proteins. Multiple genes, alternative splicing, and differential expression in brain and development.  |  Lim, S., et al. 1999. J Biol Chem. 274: 29510-8. PMID: 10506216
2. The Shank family of scaffold proteins.  |  Sheng, M. and Kim, E. 2000. J Cell Sci. 113 (Pt 11): 1851-6. PMID: 10806096
3. The G protein-coupled receptor CL1 interacts directly with proteins of the Shank family.  |  Tobaben, S., et al. 2000. J Biol Chem. 275: 36204-10. PMID: 10958799
4. Regulation of dendritic spine morphology and synaptic function by Shank and Homer.  |  Sala, C., et al. 2001. Neuron. 31: 115-30. PMID: 11498055
5. ProSAP/Shank proteins - a family of higher order organizing molecules of the postsynaptic density with an emerging role in human neurological disease.  |  Boeckers, TM., et al. 2002. J Neurochem. 81: 903-10. PMID: 12065602
6. The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.  |  Park, E., et al. 2003. J Biol Chem. 278: 19220-9. PMID: 12626503
7. SHANK1 and autism spectrum disorders.  |  Gong, X. and Wang, H. 2015. Sci China Life Sci. 58: 985-90. PMID: 26335738
8. SHANK1 is differentially expressed during development in CA1 hippocampal neurons and astrocytes.  |  Collins, SM., et al. 2018. Dev Neurobiol. 78: 363-373. PMID: 29218848
9. The roles of SHANK1 in the development of colon cancer.  |  Wang, L., et al. 2020. Cell Biochem Funct. 38: 669-675. PMID: 32356303
10. Truncating variants in the SHANK1 gene are associated with a spectrum of neurodevelopmental disorders.  |  May, HJ., et al. 2021. Genet Med. 23: 1912-1921. PMID: 34113010
11. A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.  |  Qin, Y., et al. 2022. Mol Psychiatry. 27: 2985-2998. PMID: 35388181
12. SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2.  |  Chen, B., et al. 2022. Cell Death Dis. 13: 403. PMID: 35468874

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Shank 1 Antibody (E-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Shank 1 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Shank 1 Antibody (E-1) Specifications

Alternate Product Name: SHANK1

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1701-1830

Epitope Target: Shank 1

Epitope Species: human

Product Reactivity/Detection: Shank 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Shank 1 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Shank 1/2/3 antibody is Shank 1/2/3 Antibody (G-12): sc-393963.