caspase-8 Antibody (1.1.40): sc-81656

caspase-8 Antibody (1.1.40) is a mouse monoclonal IgG1 kappa light chain antibody that detects caspase-8 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). anti-caspase-8 antibody (1.1.40) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-8 plays a crucial role in the apoptotic pathway, acting as an initiator caspase that activates downstream effector caspases by cleaving their inactive precursors. This activation is essential for the programmed cell death process, particularly in response to signals from tumor necrosis factor receptor superfamily members such as TNFRSF1A and TNFRSF6/FAS. Caspase-8 is primarily located in the cytoplasm, where caspase-8 forms a complex with the adaptor molecule FADD, leading to the formation of the death-inducing signaling complex (DISC). This complex is vital for caspase-8 activation, which subsequently dissociates and activates other apoptotic proteases, thereby propagating the apoptotic signal. Caspase-8 is widely expressed, with the highest levels found in peripheral blood leukocytes, thymus, liver, and spleen. Defects in the CASP8 gene, which encodes for caspase-8, can lead to caspase-8 deficiency disorder (CASP8D), a condition characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, potentially resulting in immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-8 Antibody (1.1.40) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-8 Antibody (1.1.40) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-8 Antibody (1.1.40) Specifications

Alternate Product Name: CASP8

Product Clone Name: 1.1.40

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: caspase-8

Epitope Species: human

Product Reactivity/Detection: caspase-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for caspase-8 Antibody (1.1.40): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.