ZNF574 Activators
ZNF574 can influence its state through a variety of molecular signaling pathways that modulate protein phosphorylation. Forskolin, for instance, targets adenylyl cyclase, leading to a rise in intracellular cAMP levels. This surge in cAMP can activate protein kinase A (PKA), which may phosphorylate ZNF574 or proteins within its regulatory complex, altering its activity state. Similarly, dibutyryl-cAMP, a cAMP analog, can permeate cellular membranes and engage cAMP-dependent pathways, potentially leading to the phosphorylation and subsequent activation of ZNF574. Isoproterenol, another chemical in this category, serves as a beta-adrenergic agonist and can also raise cAMP levels, further paving the way for PKA activation and the phosphorylation of ZNF574 or its modulatory proteins.
Ionomycin and Thapsigargin operate through calcium-dependent mechanisms, where ionomycin increases intracellular calcium levels directly, and thapsigargin does so indirectly by inhibiting the SERCA pump. Elevated calcium levels can activate calcium-dependent protein kinases, which in turn may phosphorylate ZNF574. Phorbol 12-myristate 13-acetate (PMA) and Bryostatin 1 are known to modulate protein kinase C (PKC), which is implicated in the phosphorylation of a wide range of target proteins, including those associated with ZNF574. PMA acts as a direct activator of PKC, while Bryostatin 1 modulates PKC activity, both potentially influencing ZNF574 activity. Insulin and Epidermal Growth Factor (EGF) trigger their respective receptors, setting off cascades that involve the activation of PI3K/Akt and MAPK/ERK pathways. These cascades have the capacity to phosphorylate proteins that interact with ZNF574, thus affecting its activity. Furthermore, chemicals like Anisomycin, Okadaic Acid, and Calyculin A impact ZNF574 through their actions on stress-activated and phosphatase pathways. Anisomycin activates the SAPK/JNK pathway, while Okadaic Acid and Calyculin A inhibit phosphatases like PP1 and PP2A, leading to an increased phosphorylation state of cellular proteins, which may include those associated with ZNF574 regulation. The collective action of these chemicals can modulate the phosphorylation landscape within the cell, impacting ZNF574's activation state.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium-dependent protein kinases that may phosphorylate ZNF574, leading to its activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC), which could phosphorylate ZNF574 directly or target proteins that interact with ZNF574, resulting in its activation. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin can activate phosphoinositide 3-kinase (PI3K), leading to Akt activation, which may then phosphorylate substrates that interact with ZNF574, leading to its activation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a potent activator of the stress-activated protein kinase/jun-amino-terminal kinase (SAPK/JNK) pathway, which may phosphorylate proteins that regulate ZNF574 activity, leading to its activation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid inhibits protein phosphatases PP1 and PP2A, leading to increased phosphorylation of proteins, which could include those that associate with or regulate ZNF574, resulting in its activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, similar to okadaic acid, is an inhibitor of protein phosphatases and can lead to hyperphosphorylation of proteins, potentially affecting ZNF574 activation state. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates cAMP-dependent pathways, potentially leading to changes in phosphorylation patterns that activate ZNF574. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that increases cAMP levels in the cell, which may lead to activation of protein kinase A (PKA) and subsequent phosphorylation and activation of ZNF574 or its regulators. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin raises intracellular calcium levels by inhibiting the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), potentially leading to activation of calcium-dependent kinases that could activate ZNF574. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $245.00 | 9 | |
Bryostatin 1 binds to and modulates protein kinase C (PKC), which could result in altered phosphorylation and activation of ZNF574 or its associated proteins. | ||||||