WDR43 Activators
Chemical activators of WDR43 can lead to its functional activation through various biochemical pathways, primarily involving phosphorylation, which is a common post-translational modification that regulates the activity of many proteins. Forskolin, by increasing cAMP levels, activates protein kinase A (PKA), which can phosphorylate target proteins, thereby modulating their function. This pathway can activate WDR43 by enhancing its role in ribosome biogenesis and mRNA export. Similarly, dibutyryl-cAMP, a cAMP analog, directly stimulates PKA, possibly leading to the phosphorylation and activation of WDR43. Phorbol 12-myristate 13-acetate (PMA) and its analog 12-O-Tetradecanoylphorbol-13-acetate (TPA) activate protein kinase C (PKC), which also phosphorylates proteins, potentially influencing WDR43's interaction with the RNA processing machinery. Okadaic acid and Calyculin A inhibit protein phosphatases, which normally reverse phosphorylation, thereby potentially maintaining WDR43 in an active, phosphorylated state.
Intracellular calcium levels are another regulator of protein function, and several chemical activators manipulate calcium signaling to affect WDR43. Ionomycin and A23187 increase intracellular calcium, which activates calmodulin-dependent kinases (CaMK). These kinases can activate WDR43 by modulating its interaction with the spliceosome. Thapsigargin, by inhibiting the SERCA pump, and BAY K8644, by activating L-type calcium channels, both lead to increased intracellular calcium levels, which can activate kinases capable of phosphorylating WDR43, enhancing its function in ribosome biogenesis. Ryanodine modulates ryanodine receptors to increase intracellular calcium, potentially leading to kinase activation and subsequent phosphorylation of WDR43. Anisomycin activates stress-activated protein kinases that could phosphorylate proteins interacting with WDR43, thereby influencing its role in RNA metabolism. These diverse chemical activators, through their distinct mechanisms, converge on the phosphorylation and modulation of WDR43's activity in RNA processing pathways.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Increases intracellular calcium, which activates calmodulin-dependent protein kinases (CaMK). CaMK can phosphorylate various proteins, potentially leading to the functional activation of WDR43 by affecting its interaction with the spliceosome. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Activates PKC which can phosphorylate serine and threonine residues on various proteins. This could lead to the functional activation of WDR43 by altering its interaction with components of the RNA processing machinery. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Inhibits serine/threonine protein phosphatases, which could maintain WDR43 in a phosphorylated state, leading to its continuous activation and promoting its role in RNA splicing and ribonucleoprotein complex biogenesis. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to okadaic acid, inhibits protein phosphatases leading to sustained phosphorylation of proteins. This could result in the activation of WDR43 by preventing its dephosphorylation, thereby maintaining its active role in pre-mRNA processing. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Activates stress-activated protein kinases which could phosphorylate proteins that interact with WDR43, resulting in its activation and the promotion of its function in RNA metabolism. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Inhibits the SERCA, leading to increased cytosolic calcium levels that activate calcium-dependent kinases, potentially leading to the activation of WDR43 by enhancing its interaction with the spliceosome and other components of the gene expression machinery. | ||||||
(±)-Bay K 8644 | 71145-03-4 | sc-203324 sc-203324A sc-203324B | 1 mg 5 mg 50 mg | $84.00 $196.00 $817.00 | ||
Activates L-type calcium channels, resulting in increased intracellular calcium, which can activate kinases that may phosphorylate and thereby activate WDR43, enhancing its role in ribosome biogenesis. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
A cAMP analog that directly activates PKA, potentially leading to the phosphorylation and activation of WDR43, promoting its function in mRNA processing and ribonucleoprotein complex assembly. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A calcium ionophore that increases intracellular calcium, potentially leading to the activation of WDR43 through calcium-dependent phosphorylation pathways, affecting RNA splicing and export. | ||||||
Ryanodine | 15662-33-6 | sc-201523 sc-201523A | 1 mg 5 mg | $223.00 $799.00 | 19 | |
Binds to and modulates ryanodine receptors, which can lead to an increase in intracellular calcium levels, thereby activating kinases that could phosphorylate and activate WDR43, influencing its function in RNA metabolism. | ||||||