TIP120B Activators
TIP120B, also known as CAND1 (Cullin-associated and neddylation-dissociated 1), plays a pivotal role in the regulation of the ubiquitin-proteasome system, a fundamental pathway for protein degradation critical for cellular homeostasis, signaling, and regulation. It primarily interacts with cullin-RING ubiquitin ligases (CRLs), a family of E3 ubiquitin ligases that target specific substrates for ubiquitination and subsequent proteasomal degradation. By modulating the assembly and disassembly of CRL components, TIP120B influences the ubiquitination process, affecting numerous cellular processes including cell cycle progression, signal transduction, and gene expression. Its activity and regulation are thus central to maintaining cellular function and response to environmental cues.
The activation and function of TIP120B are intricately linked to its interaction with the neddylation cycle of Cullins, a post-translational modification that adds the ubiquitin-like molecule NEDD8 to cullins to activate CRLs. TIP120B acts as a regulatory factor in this cycle by binding to unneddylated cullins, preventing their premature association with substrate receptors and adaptor molecules. This interaction is crucial for the fidelity and specificity of the ubiquitination process. Upon neddylation, TIP120B dissociates from cullins, allowing them to assemble with other CRL components and become active in substrate ubiquitination. Additionally, TIP120B's ability to disassemble CRL complexes post-ubiquitination ensures a dynamic regulation of the ubiquitination machinery, allowing for a rapid response to changing cellular environments. This dynamic regulation not only ensures the timely degradation of proteins but also the prevention of aberrant or excessive ubiquitination, highlighting the role of TIP120B in the fine-tuning of cellular processes through its activation and interaction with the ubiquitin-proteasome system.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly activates adenylate cyclase, leading to an increase in intracellular cAMP levels. Elevated cAMP activates PKA, which can phosphorylate proteins and potentially enhance the activity of TIP120B by modifying its interaction with ubiquitinated proteins. | ||||||
Spermine | 71-44-3 | sc-212953A sc-212953 sc-212953B sc-212953C | 1 g 5 g 25 g 100 g | $60.00 $192.00 $272.00 $883.00 | 1 | |
Spermine is a polyamine that stabilizes DNA and can enhance protein-protein interactions. As TIP120B is involved in the regulation of protein degradation, the presence of spermine may enhance the binding capacity of TIP120B to its targets. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc acts as a cofactor for many enzymes and is essential for the function of a multitude of proteins. It may enhance TIP120B activity by facilitating proper protein folding or stabilizing its structure, thereby enhancing its function in protein degradation control. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that raises intracellular calcium levels. Increased calcium may influence the activity of calcium-dependent proteins, indirectly enhancing TIP120B activity if TIP120B is modulated by calcium-dependent signaling pathways. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is a PKC activator that could indirectly enhance TIP120B activity by modulating signaling pathways that influence protein degradation and ubiquitination processes in which TIP120B is involved. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium ions can inhibit GSK-3, a kinase involved in various signaling pathways. Inhibition of GSK-3 might indirectly enhance TIP120B activity by affecting pathways that regulate the ubiquitination process. | ||||||
Adenosine 3′,5′-cyclic Monophosphate, N6-Benzoyl-, Sodium Salt | 30275-80-0 | sc-300167 | 10 µmol | $318.00 | 1 | |
6-Bnz-cAMP is a cAMP analog that activates PKA. Activation of PKA might indirectly enhance TIP120B function by phosphorylating proteins that interact with TIP120B, thereby influencing its role in ubiquitin-mediated degradation. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP is another cAMP analog that activates PKA. It can indirectly enhance TIP120B activity by phosphorylating proteins within pathways that TIP120B may regulate, particularly those involved in protein ubiquitination. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine is a DNA methyltransferase inhibitor that can lead to DNA demethylation. It might enhance TIP120B activity indirectly by affecting gene expression patterns of proteins that interact with or regulate TIP120B. | ||||||