Spc25 Inhibitors
Spc25 inhibitors are a class of chemical compounds specifically designed to target and inhibit the function of Spc25, a critical component of the Ndc80 complex, which is essential for proper chromosome segregation during cell division. Spc25, along with other proteins in the Ndc80 complex, such as Ndc80, Nuf2, and Spc24, plays a vital role in forming stable attachments between microtubules of the mitotic spindle and the kinetochores, which are protein structures on chromosomes that mediate chromosome movement during mitosis. The Ndc80 complex acts as a crucial linker between microtubules and kinetochores, ensuring that chromosomes are accurately aligned and segregated to daughter cells during cell division. By inhibiting Spc25, researchers can disrupt the integrity of this complex, leading to defects in chromosome alignment and segregation, which can provide valuable insights into the mechanisms of mitosis and the regulation of the cell cycle.
In research settings, Spc25 inhibitors are powerful tools for exploring the molecular mechanisms by which the Ndc80 complex ensures proper chromosome segregation and the broader implications of disrupting this process on cellular function and genome stability. By blocking Spc25 activity, scientists can investigate how the inhibition affects the attachment of microtubules to kinetochores, the dynamics of the mitotic spindle, and the fidelity of chromosome segregation. This inhibition allows researchers to study the downstream effects on cell division, including potential errors in chromosome number (aneuploidy) and the activation of cell cycle checkpoints. Additionally, Spc25 inhibitors provide insights into the interactions between Spc25 and other components of the kinetochore-microtubule interface, shedding light on the complex regulatory networks that ensure accurate chromosome segregation during mitosis. Through these studies, the use of Spc25 inhibitors enhances our understanding of the critical role of kinetochore-microtubule attachments in maintaining genomic stability, the regulation of the cell cycle, and the broader implications of these processes for cellular health and function.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $149.00 $470.00 $620.00 $1199.00 $2090.00 | 33 | |
By altering acetylation patterns on histones, Trichostatin A could lead to a compact chromatin state that hinders the transcriptional activation of the Spc25 gene. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
This agent could potentially induce DNA demethylation that may result in the silencing of genes responsible for the transcription factors that drive Spc25 gene expression. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $73.00 $238.00 $717.00 $2522.00 $21420.00 | 53 | |
By binding to DNA, Actinomycin D might block the elongation phase of RNA synthesis, leading to a decrease in Spc25 mRNA synthesis. | ||||||
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $40.00 $82.00 $256.00 | 127 | |
By halting peptide elongation during translation, Cycloheximide could drastically reduce the synthesis of the Spc25 protein. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
This compound could downregulate Spc25 expression by inhibiting the mTOR signaling pathway, which is crucial for the translation of many mRNA transcripts, including those coding for cell cycle proteins. | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $173.00 $418.00 | 43 | |
Doxorubicin could disrupt transcriptional processes by intercalating into DNA, potentially causing a reduction in Spc25 mRNA levels due to transcriptional stalling. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $65.00 $99.00 $140.00 | 85 | |
By forming DNA adducts and crosslinks, Mitomycin C might inhibit the transcription of the Spc25 gene, leading to a decrease in protein levels. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $57.00 $182.00 $92.00 | 21 | |
Camptothecin can lead to DNA damage by inhibiting topoisomerase I, which could result in the downregulation of Spc25 gene expression due to the activation of DNA damage response pathways. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine may interfere with the replication and transcription of DNA, potentially leading to a decrease in Spc25 mRNA levels. | ||||||
(±)-JQ1 | 1268524-69-1 | sc-472932 sc-472932A | 5 mg 25 mg | $226.00 $846.00 | 1 | |
By binding to BET bromodomains, JQ1 could suppress the expression of genes through chromatin remodeling, which might include the downregulation of the Spc25 gene. | ||||||