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Trichostatin A (CAS 58880-19-6)

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Alternate Names:
Trichostatin A is also known as TSA.
Application:
Trichostatin A is a potent and non-competitive reversible inhibitor of HDAC in LNCaP human prostate cancer cells, as well as in MON and HeLa cells.
CAS Number:
58880-19-6
Purity:
≥98%
Molecular Weight:
302.37
Molecular Formula:
C17H22N2O3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Trichostatin A is a potent and noncompetitive reversible inhibitor of HDAC (histone deacetylase) with a Ki of 3.4 nM. In HeLa cells, Trichostatin A blocked cell cycle progression at G1 and induced a 12-fold increase in intracellular levels of gelsolin. In cells latently infected with HIV-1, Trichostatin A induced the transcriptional activation of the HIV-1 promoter, which resulted in a marked increase in virus production. In NIH 3T3 cells, Trichostatin A induced reversion of oncogenic ras-transformed cells to a normal morphology. In Jurkat cells, it inhibited IL-2 gene expression. It also increased acetylation of GATA4, a cardiac-specific transcription factor and increases cardiac muscle cell differentiation. In normal rat fibroblasts, Trichostatin A induced Friend cell differentiation and inhibited the G1 and G2 phases of the cell cycle. It is a useful tool for induction of hyperacetylation of cellular histones and for further elucidation of their role in gene expression.


Trichostatin A (CAS 58880-19-6) References

  1. Butyrate and trichostatin A effects on the proliferation/differentiation of human intestinal epithelial cells: induction of cyclin D3 and p21 expression.  |  Siavoshian, S., et al. 2000. Gut. 46: 507-14. PMID: 10716680
  2. Trichostatin A-like hydroxamate histone deacetylase inhibitors as therapeutic agents: toxicological point of view.  |  Vanhaecke, T., et al. 2004. Curr Med Chem. 11: 1629-43. PMID: 15180568
  3. Acetylation of GATA-4 is involved in the differentiation of embryonic stem cells into cardiac myocytes.  |  Kawamura, T., et al. 2005. J Biol Chem. 280: 19682-8. PMID: 15764815
  4. Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.  |  Kishigami, S., et al. 2006. Biochem Biophys Res Commun. 340: 183-9. PMID: 16356478
  5. Histone deacetylase inhibitor trichostatin a potentiates doxorubicin-induced apoptosis by up-regulating PTEN expression.  |  Pan, L., et al. 2007. Cancer. 109: 1676-88. PMID: 17330857
  6. Genome-wide transcriptional response to 5-aza-2'-deoxycytidine and trichostatin a in multiple myeloma cells.  |  Heller, G., et al. 2008. Cancer Res. 68: 44-54. PMID: 18172295
  7. Zn(II)-dependent histone deacetylase inhibitors: suberoylanilide hydroxamic acid and trichostatin A.  |  Codd, R., et al. 2009. Int J Biochem Cell Biol. 41: 736-9. PMID: 18725319
  8. Effects of alcohol on histone deacetylase 2 (HDAC2) and the neuroprotective role of trichostatin A (TSA).  |  Agudelo, M., et al. 2011. Alcohol Clin Exp Res. 35: 1550-6. PMID: 21447001
  9. Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A.  |  Yoshida, M., et al. 1990. J Biol Chem. 265: 17174-9. PMID: 2211619
  10. How Does Chirality Determine the Selective Inhibition of Histone Deacetylase 6? A Lesson from Trichostatin A Enantiomers Based on Molecular Dynamics.  |  Zhang, Y., et al. 2019. ACS Chem Neurosci. 10: 2467-2480. PMID: 30784262
  11. Trichostatin A inhibits both ras-induced neurite outgrowth of PC12 cells and morphological transformation of NIH3T3 cells.  |  Futamura, M., et al. 1995. Oncogene. 10: 1119-23. PMID: 7700637
  12. Trichostatin A induces morphological changes and gelsolin expression by inhibiting histone deacetylase in human carcinoma cell lines.  |  Hoshikawa, Y., et al. 1994. Exp Cell Res. 214: 189-97. PMID: 8082721
  13. Transcriptional activation and chromatin remodeling of the HIV-1 promoter in response to histone acetylation.  |  Van Lint, C., et al. 1996. EMBO J. 15: 1112-20. PMID: 8605881
  14. Selective inhibition of IL-2 gene expression by trichostatin A, a potent inhibitor of mammalian histone deacetylase.  |  Takahashi, I., et al. 1996. J Antibiot (Tokyo). 49: 453-7. PMID: 8682722

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Trichostatin A, 1 mg

sc-3511
1 mg
$149.00

Trichostatin A, 5 mg

sc-3511A
5 mg
$470.00

Trichostatin A, 10 mg

sc-3511B
10 mg
$620.00

Trichostatin A, 25 mg

sc-3511C
25 mg
$1199.00

Trichostatin A, 50 mg

sc-3511D
50 mg
$2090.00

For how long is Trichostatin A stable when kept at 37° C, dissolved in DMSO?

Asked by: SCM4
While we do not have information regarding stability at 37°C in DMSO, the product is stable for 2 years at -20°C in powder form, for 2 weeks at 4°C when dissolved in DMSO, and for 6 months at -80°C when dissolved in DMSO.
Answered by: Tech Service 11
Date published: 2017-03-02
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Rated 5 out of 5 by from It has been demonstrated (PubMed ID 2211619)It has been demonstrated (PubMed ID 2211619) that Trichostatin A (TSA) causes a significant increase in histone acetylation in various mammalian cell lines: 3Y1, W3Y, DS19, HeLa and F9. -SCBT Publication Review
Date published: 2015-06-13
Rated 5 out of 5 by from One publication (PubMed ID 7700637) reportsOne publication (PubMed ID 7700637) reports that TSA does have an affect on an early stage in the NGF-signaling pathway, which is governed by ras. PC12, NIH/3T3 cells were used. -SCBT Publication Review
Date published: 2015-06-13
Rated 5 out of 5 by from An increase in expression of acetylated AcAn increase in expression of acetylated Ac-Histone H4 was observed after treatment with Trichostatin A, catalog # sc-3511 by Western Blot analysis. NIH/3T3 cells were treated with Trichostatin A prior to lysis. -SCBT QC
Date published: 2015-05-07
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Trichostatin A is rated 5.0 out of 5 by 3.
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