SEC16A Inhibitors
Chemical inhibitors of SEC16A serve to disrupt the normal function of this protein, which is essential for the export of secretory proteins from the endoplasmic reticulum (ER). Exo1, for instance, directly targets SEC16A, leading to an inhibition of the protein's function by interfering with the formation of ER exit sites, crucial for the sorting and secretion of proteins. Brefeldin A takes a slightly different approach by impairing protein transport between the ER and the Golgi apparatus; this action results in the failure of transport vesicles to form at the ER exit sites, which is a process that depends on the proper function of SEC16A. Similarly, Golgicide A disrupts the function of SEC16A by targeting Golgi BFA resistance factor 1 (GBF1), an enzyme that is indispensable for maintaining Golgi structure and function, which in turn is necessary for the proper trafficking of cargo that SEC16A helps to regulate.
Other chemicals affect SEC16A indirectly by altering the environment or the cellular structures required for its function. Monensin, as an ionophore, disrupts intracellular pH and ion gradients, which can lead to a functional inhibition of SEC16A due to the protein's reliance on these gradients for vesicle formation. Tunicamycin's inhibition of N-linked glycosylation results in an accumulation of misfolded glycoproteins, which can impair SEC16A function by blocking the formation and secretion of vesicles. Thapsigargin, by depleting ER calcium stores, disrupts calcium homeostasis, which is critical for SEC16A's role in vesicle trafficking. Agents that affect the cytoskeleton, such as Nocodazole, Cytochalasin D, Colchicine, Latrunculin A, and Paclitaxel, interfere with microtubule and actin filament dynamics, essential components for vesicle movement and stability, thus inhibiting the function of SEC16A in vesicle formation and trafficking. Lastly, Dynasore, by inhibiting the GTPase dynamin, affects clathrin-mediated endocytosis and vesicular trafficking pathways that are necessary for SEC16A's proper function. Each of these chemical inhibitors, through their unique mechanisms, can impair the ability of SEC16A to facilitate the secretion pathway, highlighting the protein's central role in cellular export processes.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Exo1 | 461681-88-9 | sc-200752 sc-200752A | 10 mg 50 mg | $82.00 $291.00 | 3 | |
Exo1 inhibits SEC16A by interfering with the export of secretory proteins from the endoplasmic reticulum (ER), which is a process SEC16A is crucial for. SEC16A is involved in the formation of ER exit sites where cargo is sorted for secretion. By disrupting this pathway, Exo1 directly inhibits SEC16A function. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
Brefeldin A targets the protein transport between the ER and the Golgi apparatus, where SEC16A has a critical role in vesicle formation. By disrupting this transport, Brefeldin A functionally inhibits SEC16A by preventing the formation of transport vesicles at the ER exit sites. | ||||||
Monensin A | 17090-79-8 | sc-362032 sc-362032A | 5 mg 25 mg | $152.00 $515.00 | ||
Monensin acts as an ionophore that disrupts intracellular pH and ion gradients. This disruption can inhibit SEC16A as it relies on proper pH and ion gradients for the formation of transport vesicles at the ER exit sites. Consequently, Monensin can cause a functional inhibition of SEC16A's role in vesicle trafficking. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation in the ER. Since SEC16A is involved in the trafficking of glycoproteins from the ER, the inhibition of this glycosylation process can result in the functional inhibition of SEC16A due to the accumulation of misfolded glycoproteins, impairing vesicle formation and secretion. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to depletion of ER calcium stores. SEC16A-dependent trafficking is sensitive to calcium levels, thus the disruption of calcium homeostasis can inhibit the function of SEC16A in the ER exit site formation and protein trafficking. | ||||||
Golgicide A | 1005036-73-6 | sc-215103 sc-215103A | 5 mg 25 mg | $187.00 $670.00 | 11 | |
Golgicide A disrupts Golgi apparatus function by inhibiting the activity of Golgi BFA resistance factor 1 (GBF1), which is essential for maintaining Golgi structure and function. Since SEC16A is involved in cargo trafficking to the Golgi, the disruption of Golgi function can lead to functional inhibition of SEC16A. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $58.00 $83.00 $140.00 $242.00 | 38 | |
Nocodazole disrupts microtubule polymerization, which is critical for vesicle transport in cells. Microtubule dynamics are essential for the proper function of SEC16A in vesicle formation and trafficking. The inhibition of microtubule function can thus inhibit SEC16A's role in the secretion pathway. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $145.00 $442.00 | 64 | |
Cytochalasin D inhibits actin polymerization, which is crucial for maintaining the cytoskeleton structure and vesicle movement. As SEC16A is involved in vesicle formation at ER exit sites, the disruption of the cytoskeleton can inhibit the function of SEC16A by impairing vesicle trafficking processes. | ||||||
Colchicine | 64-86-8 | sc-203005 sc-203005A sc-203005B sc-203005C sc-203005D sc-203005E | 1 g 5 g 50 g 100 g 500 g 1 kg | $98.00 $315.00 $2244.00 $4396.00 $17850.00 $34068.00 | 3 | |
Colchicine binds to tubulin, preventing microtubule assembly, which is necessary for vesicle and organelle transport. Since SEC16A's function is tied to vesicle trafficking, colchicine's action on microtubules leads to functional inhibition of SEC16A by disrupting transport processes that SEC16A facilitates. | ||||||
Dynamin Inhibitor I, Dynasore | 304448-55-3 | sc-202592 | 10 mg | $87.00 | 44 | |
Dynasore is a GTPase inhibitor that targets dynamin, affecting clathrin-mediated endocytosis and vesicular trafficking. This inhibition can impact SEC16A indirectly by disrupting the endocytic recycling pathways that are necessary for the proper function of SEC16A in the secretion pathway. | ||||||