PIWIL1 Activators
PIWIL1 activators encompass a class of chemical compounds designed to modulate the activity of PIWIL1, a member of the Argonaute family of proteins that plays a crucial role in gene regulation through the RNA interference (RNAi) pathway. PIWIL1, also known as PIWI-like protein 1, is particularly noted for its involvement in the biogenesis of a class of small non-coding RNAs known as PIWI-interacting RNAs (piRNAs). These activators interact with PIWIL1 to influence its function, which is integral to the maintenance of genomic stability and the regulation of gene expression, primarily in the germline cells of organisms. The chemical compounds that serve as PIWIL1 activators are typically characterized by their ability to enhance or modify the interaction between PIWIL1 and its associated piRNAs or other protein partners within the piRNA pathway.
The development and study of PIWIL1 activators are grounded in a comprehensive understanding of molecular biology and biochemistry. These molecules may be designed to bind directly to PIWIL1, altering its structural configuration and affecting its affinity for piRNAs or other interacting molecules. Alternatively, they may function indirectly, by modulating the expression levels of PIWIL1 or its cofactors, thereby impacting the piRNA pathway. The precise action of these activators can shed light on the complex mechanisms of post-transcriptional gene regulation mediated by the piRNA pathway. By selectively activating PIWIL1, researchers aim to unravel the nuances of piRNA-mediated gene silencing and the preservation of genomic integrity, which is a fundamental aspect of cellular biology, particularly in the context of germline development and function.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin activates adenylate cyclase, leading to increased cAMP levels which can enhance protein kinase A (PKA) activity. PKA can phosphorylate various proteins that may interact with PIWIL1, enhancing its RNA silencing activity. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
Rolipram is a selective inhibitor of phosphodiesterase 4 (PDE4), leading to elevated cAMP levels in cells, which could enhance PKA activity. PKA potentially phosphorylates factors that modulate PIWIL1's function in the piRNA pathway. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $105.00 $250.00 | 8 | |
Zaprinast inhibits PDE5 leading to increased cGMP levels, which may enhance the activity of protein kinase G (PKG). PKG can phosphorylate molecular targets that indirectly interact with PIWIL1, potentially enhancing its function in gene silencing. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore, which increases intracellular calcium levels, potentially activating calcium-dependent kinases that might phosphorylate proteins indirectly associated with PIWIL1, enhancing its activity. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 also acts as a calcium ionophore, raising intracellular calcium and potentially activating pathways which could lead to the phosphorylation of proteins involved in PIWIL1's RNA-induced silencing complex (RISC) loading. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 and 2A, which can lead to increased phosphorylation levels of proteins within the cell, potentially affecting those which interact with PIWIL1, enhancing its activity. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which might phosphorylate proteins that associate with or regulate PIWIL1, potentially enhancing its role in piRNA-mediated gene silencing. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG, a polyphenol in green tea, has been shown to inhibit certain kinases, which could lead to a shift in cellular signaling pathways, indirectly affecting proteins that modulate PIWIL1 activity. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor which could alter downstream AKT signaling, potentially affecting the phosphorylation state of proteins involved in the PIWIL1-mediated gene silencing process. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $90.00 $349.00 | 284 | |
SB203580 is a p38 MAPK inhibitor, which may shift cellular response to stress and influence the phosphorylation of proteins involved with PIWIL1, potentially enhancing its function in genomic stability. | ||||||