NKPD1 Activators
NKPD1 play a pivotal role in modulating its activity through various biochemical pathways. Phorbol 12-myristate 13-acetate directly influences the activation of NKPD1 by engaging protein kinase C (PKC). By activating PKC, a cascade of phosphorylation events is initiated, which includes the potential regulation and activation of NKPD1. Similarly, Forskolin raises intracellular cyclic AMP (cAMP) levels through the stimulation of adenylyl cyclase, thereby activating protein kinase A (PKA). PKA then phosphorylates proteins within cellular pathways that converge on NKPD1 activation. Ionomycin, by forming complexes with calcium ions, prompts a surge in intracellular calcium concentration that activates calcium-dependent protein kinases, which may subsequently phosphorylate and activate NKPD1.
Retinoic acid targets nuclear retinoic acid receptors to regulate gene expression, which may lead to post-translational modifications of proteins, including NKPD1, resulting in its activation. Epidermal Growth Factor (EGF) triggers its receptor tyrosine kinase, setting off a signaling cascade that can phosphorylate proteins that interact with or regulate NKPD1. Insulin, by activating its receptor, stimulates the PI3K/Akt signaling pathway, influencing a plethora of proteins, some of which are likely to interact with or directly activate NKPD1. Inhibitors such as Calyculin A and Okadaic Acid impede the action of protein phosphatases 1 and 2A, which leads to a hyperphosphorylated state within the cell, potentially affecting proteins associated with NKPD1 activation. Anisomycin, through its inhibition of protein synthesis, activates stress response pathways, including JNK and p38 MAP kinase pathways, which might lead to the activation of NKPD1. Thapsigargin disrupts calcium homeostasis, increasing cytosolic calcium levels and activating kinases that can phosphorylate NKPD1-associated proteins. Lastly, Dibutyryl-cAMP, a cAMP analog, activates PKA, which may phosphorylate and activate NKPD1 through cAMP-dependent pathways.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Directly activates protein kinase C (PKC) which, through a series of phosphorylation events, leads to the activation of NKPD1 as PKC can phosphorylate and regulate various downstream effectors involved in NKPD1 activity regulation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Increases intracellular calcium concentration by forming complexes with calcium ions, which can activate calcium-dependent protein kinases that may phosphorylate and activate NKPD1 or proteins closely linked to its regulation. | ||||||
Retinoic Acid, all trans | 302-79-4 | sc-200898 sc-200898A sc-200898B sc-200898C | 500 mg 5 g 10 g 100 g | $66.00 $325.00 $587.00 $1018.00 | 28 | |
Binds to nuclear retinoic acid receptors which can regulate gene expression and protein modification processes that lead to post-translational modifications of proteins including NKPD1, culminating in its activation. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Activates the insulin receptor, leading to the activation of the PI3K/Akt signaling pathway. This pathway can phosphorylate and activate a wide range of proteins, including those which may interact with or directly activate NKPD1. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Inhibits protein phosphatases 1 and 2A, leading to increased phosphorylation levels of cellular proteins. This inhibition can result in the hyperphosphorylation and activation of proteins in pathways that involve NKPD1. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Similarly to Calyculin A, it inhibits protein phosphatases, particularly PP1 and PP2A, which can lead to a state of increased phosphorylation of proteins within the cell, some of which may be involved in the functional activation of NKPD1. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Acts as a protein synthesis inhibitor that can activate stress response pathways such as JNK and p38 MAP kinase pathways. These pathways might lead to the activation of proteins that are part of the same signaling cascades as NKPD1, resulting in its activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Disrupts calcium storage and causes an increase in cytosolic calcium levels, which can activate calcium-dependent kinases that phosphorylate proteins within the same pathway as NKPD1, leading to its activation. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
A cell-permeable cAMP analog that activates cAMP-dependent pathways such as PKA. Activation of PKA can lead to phosphorylation of target proteins that are involved in NKPD1-related pathways, thereby activating NKPD1. | ||||||