LOC649935 Activators
Chemical activators of LOC649935 can induce its activation through various intracellular signaling pathways primarily by modulating the phosphorylation status of the protein. Forskolin, by activating adenylyl cyclase, increases intracellular cAMP levels, which in turn activate Protein Kinase A (PKA). PKA is then capable of phosphorylating LOC649935, which leads to its activation. Similarly, Dibutyryl-cAMP (db-cAMP), a cell-permeable cAMP analog, directly stimulates PKA, thereby promoting the phosphorylation and subsequent activation of LOC649935. PMA, which activates Protein Kinase C (PKC), also plays a role in the phosphorylation and activation of LOC649935, as PKC phosphorylates a variety of proteins within the cell. Anisomycin, through its ability to activate stress-activated protein kinases like JNK, can also facilitate the phosphorylation and activation of LOC649935 if it is a substrate of these kinases.
On the other hand, Ionomycin and Thapsigargin increase intracellular calcium levels, which in turn can activate calcium/calmodulin-dependent protein kinases (CaMKs). If LOC649935 is a substrate for CaMKs, the elevation in calcium levels would lead to its phosphorylation and activation. Pervanadate and Okadaic Acid, by inhibiting protein tyrosine phosphatases and protein phosphatases 1 and 2A respectively, prevent the dephosphorylation of proteins, which may include LOC649935, thus maintaining it in an active state. Similarly, Calyculin A, another potent inhibitor of protein phosphatases 1 and 2A, ensures that LOC649935 remains phosphorylated and active. Zinc Pyrithione donates zinc ions which can act as cofactors for various enzymes; if LOC649935 requires zinc for its activation or is modulated by zinc-dependent enzymes, its activation is ensured. Lastly, IBMX, by inhibiting phosphodiesterases, raises cAMP levels, which can enhance PKA activity leading to the activation of LOC649935 through phosphorylation.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates Protein Kinase C (PKC), which is known to phosphorylate a variety of proteins. PKC-mediated phosphorylation of LOC649935 would result in its activation within the cellular context where PKC directly interacts with its substrate proteins. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium/calmodulin-dependent protein kinases (CaMKs). These kinases could then phosphorylate and activate LOC649935 if it is a substrate for CaMKs. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leading to increased cytosolic calcium levels. This elevation in calcium can activate CaMKs or other calcium-dependent kinases that may phosphorylate and activate LOC649935. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is an inhibitor of protein phosphatases 1 and 2A. By inhibiting these phosphatases, Okadaic Acid prevents the dephosphorylation of proteins, potentially keeping LOC649935 in a phosphorylated and active state. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc Pyrithione donates zinc ions, which can serve as cofactors for various signaling enzymes. If LOC649935 has a zinc-dependent activation mechanism or is modulated by enzymes that require zinc, Zinc Pyrithione can activate LOC649935 by ensuring proper enzyme function. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable analog of cAMP that directly activates PKA. PKA then can phosphorylate and activate LOC649935 if it is a substrate for PKA. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is known to activate stress-activated protein kinases like JNK (c-Jun N-terminal kinases). If LOC649935 is a substrate for JNK or similar kinases, Anisomycin could result in its activation through phosphorylation by these kinases. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is a selective inhibitor of PKC, but it can lead to compensatory activation of other kinases. If LOC649935 is a substrate for such alternative kinases, Bisindolylmaleimide I could indirectly lead to the activation of LOC649935. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatase 1 and 2A, similar to Okadaic Acid. It could therefore maintain LOC649935 in a phosphorylated and active state by preventing its dephosphorylation. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, leading to an increase in cAMP levels. Higher cAMP levels can enhance PKA activity which, if LOC649935 is a substrate of PKA, would result in phosphorylation and activation of LOC649935. | ||||||