KLHL35 Activators
KLHL35 can initiate a cascade of intracellular events that lead to the protein's activation through various mechanisms. Forskolin is one such chemical that directly targets the enzyme adenylyl cyclase, thereby boosting the levels of cAMP within the cell. This increase in cAMP activates Protein Kinase A (PKA), which can then phosphorylate various substrates, including KLHL35. Similarly, IBMX raises cAMP levels but does so by inhibiting phosphodiesterases that would otherwise degrade cAMP. The outcome is an indirect yet significant enhancement of PKA activity, which again can lead to the phosphorylation and subsequent activation of KLHL35. Dibutyryl-cAMP, being a cAMP analog, bypasses the cellular mechanisms for cAMP production and directly activates PKA, thereby streamlining the phosphorylation and activation of KLHL35.
PMA mimics the action of diacylglycerol (DAG) and activates Protein Kinase C (PKC). PKC then potentially phosphorylates KLHL35, leading to its activation. Calcium ionophores like Ionomycin and A23187 elevate intracellular calcium levels, which can activate various calcium-dependent pathways. These include the activation of calcineurin or calmodulin-dependent kinase, both of which may affect the phosphorylation state of KLHL35. Thapsigargin, by inhibiting the SERCA pump, also increases cytosolic calcium, indirectly fostering an environment that facilitates the activation of KLHL35. Moreover, Okadaic Acid and Calyculin A prevent the dephosphorylation of proteins by inhibiting protein phosphatases PP1 and PP2A. This inhibition may lead to a state of hyperphosphorylation, which can include the activation of KLHL35. Anisomycin, by activating stress-activated protein kinases, can indirectly promote the activation of KLHL35 through phosphorylation. Lastly, Spermidine, through its role in modulating NMDA receptor activity and subsequent calcium influx, may influence the activation of calcium-dependent kinases and phosphatases, contributing to the activation of KLHL35.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP activates PKA (Protein Kinase A), which can phosphorylate various proteins, potentially including KLHL35, leading to its activation. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
Isobutylmethylxanthine (IBMX) is a non-specific inhibitor of phosphodiesterases, which prevents the breakdown of cAMP. This action results in increased levels of cAMP within the cell, indirectly enhancing the activation of PKA and possibly increasing phosphorylation and activation of KLHL35. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) is a diacylglycerol (DAG) analog which activates protein kinase C (PKC). PKC has been implicated in the phosphorylation of a wide range of proteins, and its activation may lead to subsequent phosphorylation and activation of KLHL35. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore which increases intracellular calcium levels and can activate calcineurin. Calcineurin dephosphorylates members of the NFAT family, leading to their nuclear translocation. NFAT may enhance the transcription of proteins that interact with and activate KLHL35. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor which leads to an increase in cytosolic calcium levels, indirectly activating pathways dependent on calcium such as calmodulin-dependent kinase, which could phosphorylate and activate KLHL35. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
Calcimycin (A23187) is another calcium ionophore, which similarly to ionomycin, increases intracellular calcium and can activate calcium-dependent proteins that could potentially lead to the activation of KLHL35. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels of proteins within the cell. This inhibition may result in the hyperphosphorylation and activation of KLHL35. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that activates stress-activated protein kinases (SAPKs), which could lead to the phosphorylation and activation of downstream proteins, potentially including KLHL35. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is also an inhibitor of protein phosphatases like okadaic acid and can similarly lead to increased phosphorylation and potential activation of proteins like KLHL35 by preventing their dephosphorylation. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that directly activates cAMP-dependent pathways including PKA. Activation of PKA could result in phosphorylation and subsequent activation of KLHL35. | ||||||