HICE1 Activators
HICE1 can initiate a variety of intracellular signaling cascades that lead to its activation. Forskolin directly stimulates adenylate cyclase, which catalyzes the conversion of ATP to cyclic AMP (cAMP). Elevated cAMP levels activate protein kinase A (PKA), which in turn can phosphorylate HICE1, leading to its activation. Similarly, Isoproterenol, a beta-adrenergic agonist, engages beta-adrenergic receptors triggering a signaling pathway that also culminates in adenylate cyclase activation, cAMP accumulation, PKA activation, and subsequent HICE1 phosphorylation. Prostaglandin E1 (PGE1) operates through its specific G-protein-coupled receptors to activate adenylate cyclase, following the same pathway to HICE1 activation. On the other hand, IBMX inhibits phosphodiesterases, preventing cAMP degradation, which indirectly sustains PKA activity and can promote HICE1 activation.
Additional chemical activators work through different mechanisms to influence HICE1 activity. Anisomycin, although primarily a protein synthesis inhibitor, activates stress-activated protein kinases like JNK, which may intersect with signaling pathways involving HICE1. Ionomycin increases intracellular calcium levels, thereby activating calcium-dependent kinases that have the capacity to phosphorylate HICE1. TPA directly activates protein kinase C (PKC), which can phosphorylate and activate a suite of proteins, potentially including HICE1. Dibutyryl-cAMP (db-cAMP) is a synthetic cAMP analog that diffuses into cells and directly activates PKA, circumventing cell surface receptors and leading to HICE1 activation. By contrast, BIM inhibits PKC but can lead to the activation of alternative pathways that may result in HICE1 activation. Rolipram inhibits PDE4, leading to increased cAMP levels and enhanced PKA activity, promoting the phosphorylation of HICE1. Ouabain alters cellular ion gradients by inhibiting the Na+/K+ ATPase, which can initiate signaling cascades affecting HICE1. Lastly, A23187, like ionomycin, is a calcium ionophore that can activate calcium-dependent signaling pathways and potentially result in HICE1 phosphorylation. Each chemical, through its unique mode of action, converges on the activation of HICE1, albeit via distinct biochemical routes.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly activates adenylate cyclase, thereby increasing levels of cAMP, which is a secondary messenger known to enhance the activity of multiple proteins, including HICE1, by phosphorylation through cAMP-dependent protein kinase A (PKA). | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that activates the beta-adrenergic receptor leading to adenylate cyclase activation. This increases cAMP in the cell, which activates PKA, subsequently leading to the activation of proteins such as HICE1 within cellular signaling pathways. | ||||||
PGE1 (Prostaglandin E1) | 745-65-3 | sc-201223 sc-201223A | 1 mg 10 mg | $30.00 $142.00 | 16 | |
Prostaglandin E1 binds to its respective G-protein-coupled receptor, leading to adenylate cyclase activation and an increase in cAMP levels, ultimately causing the activation of PKA which can phosphorylate and thereby activate proteins including HICE1. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, enzymes that break down cAMP. By preventing cAMP degradation, IBMX indirectly increases PKA activity, which can lead to the activation of proteins like HICE1 by phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that also activates stress-activated protein kinases such as JNK. The activation of JNK has been shown to influence various signaling cascades, potentially leading to the activation of proteins like HICE1 through phosphorylation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calmodulin-dependent kinases, potentially leading to the phosphorylation and activation of certain proteins, including HICE1. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
TPA activates protein kinase C (PKC), which is involved in multiple signaling pathways and can lead to the activation of many proteins through phosphorylation, including HICE1. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a membrane-permeable cAMP analog that activates PKA directly, bypassing receptor-mediated pathways. PKA activation can then result in the phosphorylation and functional activation of target proteins such as HICE1. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
BIM is a PKC inhibitor, but in certain contexts, it can paradoxically lead to the activation of alternative pathways that compensate for PKC inhibition, potentially resulting in the activation of proteins like HICE1. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $75.00 $212.00 | 18 | |
Rolipram is a selective inhibitor of phosphodiesterase 4 (PDE4), which hydrolyzes cAMP. By inhibiting PDE4, rolipram increases cAMP levels, leading to activation of PKA that may phosphorylate and activate proteins including HICE1. | ||||||