Gm757 Activators
The chemical class dubbed Ring Finger Protein 224 Activators encompasses a range of compounds that enhance the ubiquitin ligase activity of Rnf224 through various signaling pathways. Forskolin, by raising intracellular cAMP levels, indirectly promotes Rnf224's activity by potentiating PKA-mediated phosphorylation of potential substrates or regulatory proteins associated with Rnf224. Activation of PKC by PMA could lead to similar outcomes, with phosphorylated proteins presenting as targets for Rnf224-mediated ubiquitination. Ionomycin, through the elevation of intracellular calcium and subsequent activation of calmodulin-dependent kinases, could phosphorylate proteins that influence the ubiquitination process in which Rnf224 is involved.
EGF and insulin are growth factors that trigger specific kinases through the MAPK/ERK and PI3K/AKT pathways respectively, cascading into phosphorylation events that could enable Rnf224 to ubiquitinate key signaling proteins. H2O2 and sodium orthovanadate modulate the phosphorylation state of proteins either by inducing oxidative modifications or by inhibiting phosphatase activity, which could result in an increased pool of substrates for Rnf224.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a potent activator of protein kinase C (PKC). Activation of PKC can lead to phosphorylation of substrates and alteration of cellular processes that could enhance the ubiquitination activity of Rnf224 by promoting its interaction with specific substrates. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that raises intracellular calcium levels, activating calmodulin-dependent kinases. These kinases can phosphorylate proteins that may modulate the ubiquitination landscape, potentially amplifying the functional activity of Rnf224. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin triggers the PI3K/AKT pathway, which can lead to the phosphorylation and activation of downstream targets that may interact with Rnf224, possibly enhancing its role in ubiquitin-mediated signaling. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
H2O2 is a reactive oxygen species that can modulate signaling pathways through the oxidative modification of proteins. This modification can influence protein interactions and stability, potentially increasing Rnf224 activity in the elimination of damaged proteins. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
Sodium orthovanadate is an inhibitor of tyrosine phosphatases. By preventing dephosphorylation, it can sustain the phosphorylation state of proteins that might be involved in enhancing the function of Rnf224 through prolonged signaling activity. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium chloride inhibits GSK-3. Inhibition of GSK-3 can lead to the stabilization and accumulation of proteins that might be ubiquitinated by Rnf224, thereby potentially enhancing the ubiquitination process mediated by Rnf224. | ||||||
β-Nicotinamide mononucleotide | 1094-61-7 | sc-212376 sc-212376A sc-212376B sc-212376C sc-212376D | 25 mg 100 mg 1 g 2 g 5 g | $110.00 $150.00 $220.00 $300.00 $600.00 | 4 | |
NMN enhances NAD+ levels, which is a substrate for sirtuins. Sirtuins deacetylate proteins, which can affect their function and interaction with ubiquitin ligases like Rnf224, potentially enhancing Rnf224-mediated ubiquitination. | ||||||
Resveratrol | 501-36-0 | sc-200808 sc-200808A sc-200808B | 100 mg 500 mg 5 g | $80.00 $220.00 $460.00 | 64 | |
Resveratrol activates sirtuins, which can lead to deacetylation of proteins. Deacetylated proteins may be preferred substrates for Rnf224, potentially enhancing its activity in the ubiquitin-proteasome pathway. | ||||||
Curcumin | 458-37-7 | sc-200509 sc-200509A sc-200509B sc-200509C sc-200509D sc-200509F sc-200509E | 1 g 5 g 25 g 100 g 250 g 1 kg 2.5 kg | $37.00 $69.00 $109.00 $218.00 $239.00 $879.00 $1968.00 | 47 | |
Curcumin can modulate various signaling pathways, including NF-κB, which is involved in the regulation of protein turnover. This could indirectly enhance the functional activity of Rnf224 by modulating the environment in which it operates. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $63.00 $158.00 $326.00 | 233 | |
Rapamycin inhibits mTOR, which can lead to the upregulation of autophagy. Enhanced autophagy may indirectly increase the demand for ubiquitination activity, potentially enhancing Rnf224 function as it may be involved in tagging proteins for autophagic degradation. | ||||||