DNAHC12 Activators
DNAH12, encoding the dynein axonemal heavy chain 12, plays a crucial role in microtubule-based movement. Predicted to be located in cilium, cytoplasm, and microtubule, it is part of the dynein complex. The activation of DNAH12 involves intricate molecular events influenced by various chemical activators. Lithium chloride, by stabilizing β-catenin and promoting its nuclear translocation, enhances DNAH12 transcription. Forskolin elevates cAMP levels, activating PKA, which phosphorylates CREB, facilitating DNAH12 activation. Sodium butyrate, through histone deacetylase inhibition, creates a permissive chromatin state for DNAH12 transcription. Retinoic acid, binding to retinoic acid receptors, directly activates DNAH12 by enhancing its transcription. Dibutyryl cyclic AMP mimics endogenous cAMP, activating PKA and promoting DNAH12 transcription. Trichostatin A inhibits histone deacetylases, altering chromatin structure for DNAH12 activation. Dimethyl sulfoxide modulates the NF-κB pathway, preventing its inhibitory effect on DNAH12. 5-Azacytidine promotes DNA demethylation, facilitating DNAH12 transcription. Valproic acid inhibits histone deacetylases, creating a permissive chromatin environment for DNAH12 activation.
2-Aminopurine directly activates DNAH12 by inhibiting PKR, relieving its inhibitory effect on DNAH12 translation. Sodium valproate, through histone deacetylase inhibition, promotes DNAH12 transcription. Epigallocatechin gallate indirectly activates DNAH12 by inhibiting MEK in the MAPK/ERK pathway, relieving SPRY4-mediated inhibition and promoting DNAH12 transcription. This comprehensive understanding of DNAH12 activation sheds light on avenues for modulation, providing insights into its role in microtubule-based movement within cells.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium chloride enhances DNAH12 by influencing the Wnt signaling pathway. It stabilizes β-catenin, promoting its translocation into the nucleus, where it interacts with DNAH12 promoter elements, leading to increased transcription and activation. | ||||||
Sodium Butyrate | 156-54-7 | sc-202341 sc-202341B sc-202341A sc-202341C | 250 mg 5 g 25 g 500 g | $31.00 $47.00 $84.00 $222.00 | 19 | |
Sodium butyrate acts as an indirect activator by influencing histone acetylation. It inhibits histone deacetylases, leading to increased acetylation of histones associated with the DNAH12 gene, promoting a more permissive chromatin state for transcription. | ||||||
Retinoic Acid, all trans | 302-79-4 | sc-200898 sc-200898A sc-200898B sc-200898C | 500 mg 5 g 10 g 100 g | $66.00 $325.00 $587.00 $1018.00 | 28 | |
Retinoic acid acts as a direct activator by binding to retinoic acid receptors (RARs). The RAR-retinoic acid complex binds to retinoic acid response elements (RAREs) in the DNAH12 promoter, facilitating transcription and subsequent activation of DNAH12. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl cyclic AMP directly activates DNAH12 by mimicking the effects of endogenous cAMP. It activates PKA, leading to the phosphorylation of CREB, which then binds to the DNAH12 promoter, enhancing transcription and promoting DNAH12 activation. | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $152.00 $479.00 $632.00 $1223.00 $2132.00 | 33 | |
Trichostatin A acts as an indirect activator by inhibiting histone deacetylases. This leads to increased histone acetylation at the DNAH12 promoter, creating a more favorable chromatin structure for transcription and subsequent activation of DNAH12. | ||||||
Dimethyl Sulfoxide (DMSO) | 67-68-5 | sc-202581 sc-202581A sc-202581B | 100 ml 500 ml 4 L | $31.00 $117.00 $918.00 | 136 | |
DMSO indirectly activates DNAH12 by modulating the NF-κB signaling pathway. It inhibits IκB kinase, preventing IκB degradation. The stabilized IκB retains NF-κB in the cytoplasm, limiting its inhibitory effect on DNAH12 transcription and promoting its activation. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine acts as an indirect activator by promoting DNA demethylation. It inhibits DNA methyltransferases, leading to reduced DNA methylation at the DNAH12 promoter. The hypomethylated state facilitates transcription and subsequent activation of DNAH12. | ||||||
Valproic Acid | 99-66-1 | sc-213144 | 10 g | $87.00 | 9 | |
Valproic acid indirectly activates DNAH12 by inhibiting histone deacetylases. This results in increased histone acetylation at the DNAH12 promoter, creating a more permissive chromatin environment for transcription and subsequent activation of DNAH12. | ||||||
2-Aminopurine | 452-06-2 | sc-287828 sc-287828A | 100 mg 250 mg | $118.00 $185.00 | ||
2-Aminopurine directly activates DNAH12 by inhibiting protein kinase R (PKR). Inhibition of PKR prevents its inhibitory effect on DNAH12 translation, leading to increased synthesis of DNAH12 protein and subsequent activation. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG activates DNAH12 indirectly by modulating the MAPK/ERK signaling pathway. It inhibits MEK, downstream of ERK, reducing SPRY4 expression. Reduced SPRY4 levels relieve the inhibition on the pathway, promoting DNAH12 transcription and subsequent activation. | ||||||