DENND4A Inhibitors
Chemical inhibitors of DENND4A target various aspects of the cellular machinery to exert their inhibitory effects on this protein's function. Wortmannin and LY294002, both phosphoinositide 3-kinases (PI3K) inhibitors, can disrupt the PI3K signaling pathway that is pivotal for vesicle trafficking, a process where DENND4A plays a role. By impeding PI3K activity, these inhibitors can alter membrane dynamics and vesicular transport, which are crucial for DENND4A's function in the cell. Similarly, Dynasore, a GTPase inhibitor, targets dynamin, which is essential for clathrin-mediated endocytosis. Inhibition of dynamin by Dynasore leads to a breakdown in the vesicular scission from the membrane, thus hindering DENND4A's associated functions in vesicle formation and trafficking. The microtubule-affecting agents Paclitaxel (Taxol) and Nocodazole also play a role in the inhibition of DENND4A. Paclitaxel stabilizes microtubules, while Nocodazole disrupts their polymerization, both of which are critical for vesicle transport-a process that DENND4A is known to be involved in. These disruptions in microtubule dynamics can, therefore, inhibit the proper functioning of DENND4A in vesicle trafficking.
Further, compounds like Brefeldin A and Monensin, which interfere with Golgi function, can indirectly inhibit DENND4A. Brefeldin A inhibits ADP-ribosylation factor, thus disrupting ARF-dependent vesicle transport, while Monensin alters Golgi pH and ion gradients, affecting the organelle's function and, by extension, DENND4A's role in membrane trafficking. Cytochalasin D and Latrunculin A both inhibit actin polymerization, which is indispensable for vesicle movement. Their actions can impair DENND4A by obstructing vesicle motility and secretion processes that rely on an intact cytoskeleton. Tunicamycin, by inhibiting N-linked glycosylation, can also indirectly inhibit DENND4A by impeding the trafficking of proteins that are necessary for DENND4A's function. Chlorpromazine, which disrupts clathrin assembly and endocytosis, can hinder DENND4A's role in these pathways. Lastly, Vinblastine, which promotes microtubule depolymerization, can inhibit DENND4A by disrupting the cellular transport mechanisms upon which DENND4A's functions are dependent.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $66.00 $219.00 $417.00 | 97 | |
Wortmannin is a potent inhibitor of phosphoinositide 3-kinases (PI3K). DENND4A is involved in the regulation of vesicle trafficking, a process that can be influenced by PI3K signaling. By inhibiting PI3K, Wortmannin can disrupt membrane dynamics and vesicular transport, thereby functionally inhibiting DENND4A's role in these processes. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is another PI3K inhibitor, similar to Wortmannin. It prevents activation of PI3K, leading to a downstream inhibition of the vesicle trafficking processes that DENND4A is involved in, thus functionally inhibiting DENND4A's role in the cell. | ||||||
Dynamin Inhibitor I, Dynasore | 304448-55-3 | sc-202592 | 10 mg | $87.00 | 44 | |
Dynasore is a GTPase inhibitor that targets dynamin, a protein essential for clathrin-mediated endocytosis, a pathway where DENND4A has a functional role. By inhibiting dynamin, Dynasore can hinder the vesicular scission from the membrane, thereby indirectly inhibiting DENND4A's associated functions in vesicle formation and trafficking. | ||||||
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $40.00 $73.00 $217.00 $242.00 $724.00 $1196.00 | 39 | |
Paclitaxel stabilizes microtubules and prevents their disassembly, which is crucial for vesicle transport, a process in which DENND4A is involved. By stabilizing microtubules, Paclitaxel can inhibit the proper functioning of DENND4A in vesicle trafficking. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $58.00 $83.00 $140.00 $242.00 | 38 | |
Nocodazole disrupts microtubule polymerization. As microtubules are necessary for vesicle transport, the disruption caused by Nocodazole can indirectly inhibit the function of DENND4A by impeding the cellular transport mechanisms it relies upon. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
Brefeldin A is an inhibitor of ADP-ribosylation factor (ARF), a small GTPase involved in vesicle formation from the Golgi apparatus. DENND4A, being implicated in membrane trafficking, can be functionally inhibited through the disruption of ARF-dependent vesicle transport caused by Brefeldin A. | ||||||
Monensin A | 17090-79-8 | sc-362032 sc-362032A | 5 mg 25 mg | $152.00 $515.00 | ||
Monensin is an ionophore that disrupts Golgi function by altering pH and ion gradients. Given that DENND4A is involved in membrane trafficking, the alteration of Golgi dynamics by Monensin can functionally inhibit DENND4A's role in this pathway. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $145.00 $442.00 | 64 | |
Cytochalasin D inhibits actin polymerization, which is essential for maintaining cell shape and enabling vesicle movement. By disrupting actin filaments, Cytochalasin D can functionally inhibit DENND4A by hindering vesicle motility and secretion processes that require intact cytoskeleton. | ||||||
Latrunculin A, Latrunculia magnifica | 76343-93-6 | sc-202691 sc-202691B | 100 µg 500 µg | $260.00 $799.00 | 36 | |
Latrunculin A also targets actin polymerization. Similar to Cytochalasin D, by disrupting actin cytoskeleton, Latrunculin A functionally inhibits DENND4A as it impairs the cytoskeletal dynamics necessary for DENND4A's role in vesicle transport. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation in the endoplasmic reticulum. Since glycosylation is essential for proper protein folding and trafficking, Tunicamycin can indirectly inhibit DENND4A by impairing the trafficking of proteins that may be necessary for DENND4A's function in vesicle formation and transport. | ||||||