Calmegin Inhibitors
Chemical inhibitors of Calmegin can function through a variety of mechanisms that interfere with the protein's calcium-dependent chaperone activity within the endoplasmic reticulum (ER). Chelating agents such as EDTA, EGTA, BAPTA, and Phenanthroline sequester divalent cations, particularly calcium, from the cellular environment. By binding calcium ions, these chelators prevent Calmegin from accessing the calcium ions it requires for its structural stability and function. Calmegin's activity is contingent upon calcium-dependent conformational changes and interactions; thus, chelation of calcium can lead to a functional inhibition of this protein. Likewise, Ruthenium Red acts as another inhibitor by blocking calcium channels, which are crucial for maintaining the calcium influx that Calmegin needs for its activity. Thapsigargin and Cyclopiazonic Acid pose a similar effect by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump, leading to the depletion of calcium from ER stores, which in turn inhibits Calmegin by depriving it of the necessary calcium ions.
Furthermore, Tunicamycin disrupts Calmegin's function by inhibiting N-linked glycosylation, a post-translational modification that aids in the proper folding and stability of ER luminal proteins, including Calmegin. On the other hand, 2-APB alters calcium release by inhibiting IP3 receptors, thereby reducing the intracellular calcium signaling that Calmegin relies on. Ryanodine and Dantrolene both modulate the ryanodine receptors, which affects the release of calcium from the ER, and as a consequence, can inhibit Calmegin by influencing the calcium homeostasis within the ER lumen. Lastly, Bepridil, a calcium channel antagonist, impedes calcium influx, which can also disrupt the internal calcium balance and consequently, the functionality of Calmegin. Each of these chemicals, by altering the calcium-dependent pathways and processes, can lead to the functional inhibition of Calmegin, showcasing the intricate dependency of this protein on the tightly regulated ER calcium homeostasis.
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Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
1,10-Phenanthroline | 66-71-7 | sc-255888 sc-255888A | 2.5 g 5 g | $23.00 $31.00 | ||
Phenanthroline chelates metal ions necessary for the enzymatic activity of certain metalloproteases which may be involved in the same pathways or processes as Calmegin, potentially leading to a reduction in its functional activity due to altered proteolytic processing that Calmegin depends on. | ||||||
EGTA | 67-42-5 | sc-3593 sc-3593A sc-3593B sc-3593C sc-3593D | 1 g 10 g 100 g 250 g 1 kg | $20.00 $62.00 $116.00 $246.00 $799.00 | 23 | |
EGTA preferentially binds to calcium ions, which can inhibit calcium-dependent chaperones and enzymes within the endoplasmic reticulum where Calmegin operates, leading to an indirect inhibition of Calmegin's function due to the lack of essential calcium ions. | ||||||
BAPTA, Free Acid | 85233-19-8 | sc-201508 sc-201508A | 100 mg 500 mg | $67.00 $262.00 | 10 | |
BAPTA, like EGTA, is a calcium chelator that can disrupt calcium signaling. By buffering intracellular calcium, it can lead to the functional inhibition of Calmegin by preventing calcium-dependent conformational changes or interactions necessary for its activity. | ||||||
Ruthenium red | 11103-72-3 | sc-202328 sc-202328A | 500 mg 1 g | $184.00 $245.00 | 13 | |
Ruthenium Red is known to bind to calcium channels, blocking calcium influx. Inhibition of calcium influx can decrease the activity of calcium-dependent chaperones including Calmegin, leading to functional inhibition due to impaired calcium signaling. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leading to depletion of ER calcium stores. This disrupts the function of calcium-dependent proteins in the ER, such as Calmegin, by depriving them of the calcium ions required for their activity. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation, a process that can be crucial for the folding and stability of certain proteins. Calmegin, being a luminal ER protein, may require proper glycosylation for its function. Therefore, tunicamycin can lead to the functional inhibition of Calmegin by preventing its proper folding and stability. | ||||||
Cyclopiazonic Acid | 18172-33-3 | sc-201510 sc-201510A | 10 mg 50 mg | $173.00 $612.00 | 3 | |
Cyclopiazonic Acid is an inhibitor of SERCA, similar to thapsigargin, leading to a decrease in ER calcium levels. This can indirectly inhibit Calmegin by disrupting the calcium-dependent mechanisms necessary for its chaperone activity in the ER. | ||||||
2-APB | 524-95-8 | sc-201487 sc-201487A | 20 mg 100 mg | $27.00 $52.00 | 37 | |
2-APB inhibits IP3 receptors and can alter the release of calcium from the ER. Calmegin, which relies on calcium for its function, can be functionally inhibited by 2-APB due to diminished calcium signaling within the ER. | ||||||
Ryanodine | 15662-33-6 | sc-201523 sc-201523A | 1 mg 5 mg | $219.00 $765.00 | 19 | |
Ryanodine binds to and modulates ryanodine receptors which control the release of calcium from the sarcoplasmic and endoplasmic reticulum. By altering calcium release, ryanodine can indirectly inhibit Calmegin by affecting the calcium homeostasis that is crucial for Calmegin's activity. | ||||||
Dantrolene | 7261-97-4 | sc-500165 | 25 mg | $350.00 | 7 | |
Dantrolene interferes with ryanodine receptors, inhibiting the release of calcium from intracellular stores. This can indirectly inhibit Calmegin by reducing the calcium levels necessary for its function within the ER, thus affecting its chaperone activity. | ||||||