BMP-1 Activators
BMP-1 Activators are a set of compounds that can modulate the activity of BMP-1, a protein involved in the extracellular matrix formation and bone development. These activators act through a variety of mechanisms, often involving the modulation of signaling pathways that can influence the activity of BMP-1. The activators selected influence the BMP-1 signaling pathway primarily through the modulation of intracellular cAMP levels or the regulation of intracellular calcium signaling. Activators such as Forskolin, IBMX, and Rolipram raise cAMP levels, either directly (Forskolin) or by inhibiting its degradation (IBMX, Rolipram).This increase in cAMP leads to the stimulation of the Smad1/5/8 complex, which forms a complex with Smad4 and translocates into the nucleus to modulate BMP-1 gene expression. These activators exemplify compounds that can act upstream of BMP-1 and modulate its activity indirectly, through the manipulation of other cellular processes.
Other activators, such as A23187, Thapsigargin, PMA, Ionomycin, and BAPTA-AM, act through the regulation of intracellular calcium signaling. These activators either increase intracellular calcium levels directly (A23187, Thapsigargin, Ionomycin) or alter calcium signaling (BAPTA-AM), leading to the activation of calcium-dependent PKC. Activated PKC can phosphorylate and activate the transcription factor NFAT, which then translocates into the nucleus and enhances BMP-1 gene expression. In addition, some of the identified activators, such as U73122, LY294002, SB415286, and BIO, act by modulating other signaling pathways that can influence BMP-1. U73122, a PLC inhibitor, alters PKC activity and subsequently the activation of NFAT, potentially leading to changes in BMP-1 gene expression. LY294002, SB415286, and BIO all act by modulating the PI3K/Akt/GSK-3β pathway, leading to changes in β-catenin stability and its interaction with TCF/LEF transcription factors, which can modulate BMP-1 gene expression.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin is a direct activator of adenylate cyclase, an enzyme that catalyzes the conversion of ATP to cAMP. In the BMP-1 signaling pathway, elevated cAMP levels can stimulate Smad1/5/8, which then forms a complex with Smad4. This complex translocates into the nucleus, where it can modulate BMP-1 gene expression. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, the enzymes that break down cAMP. By preventing cAMP degradation, IBMX sustains elevated cAMP levels, which can stimulate the Smad1/5/8 complex and subsequently enhance BMP-1 gene expression. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
Rolipram is a selective phosphodiesterase-4 (PDE4) inhibitor. By selectively inhibiting PDE4, it prevents cAMP degradation, leading to stimulation of the Smad1/5/8 complex and enhancing BMP-1 gene expression. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 is a calcium ionophore that increases intracellular calcium levels. Elevated calcium levels can activate calcium-dependent PKC, which can phosphorylate and activate the transcription factor NFAT. Activated NFAT translocates to the nucleus and can enhance BMP-1 gene expression. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin induces calcium release from intracellular stores, increasing cytosolic calcium levels. This increase can activate calcium-dependent PKC, leading to activation of NFAT and subsequent enhancement of BMP-1 gene expression. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a potent activator of Protein Kinase C (PKC). Activated PKC can phosphorylate and activate NFAT, which then translocates to the nucleus and enhances BMP-1 gene expression. | ||||||
Ionomycin, free acid | 56092-81-0 | sc-263405 sc-263405A | 1 mg 5 mg | $96.00 $264.00 | 2 | |
Ionomycin, a calcium ionophore, increases intracellular calcium levels. The elevated calcium levels activate calcium-dependent PKC, which can phosphorylate and activate NFAT. Once activated, NFAT translocates to the nucleus and enhances BMP-1 gene expression. | ||||||
BAPTA/AM | 126150-97-8 | sc-202488 sc-202488A | 25 mg 100 mg | $138.00 $458.00 | 61 | |
BAPTA-AM is a calcium chelator that can alter intracellular calcium levels. By affecting calcium levels, BAPTA-AM can modulate the activity of calcium-dependent PKC and the subsequent activation of NFAT, which can enhance BMP-1 gene expression. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor. By inhibiting PI3K, it prevents the downstream activation of Akt, which can phosphorylate and inhibit GSK-3β. The inhibition of GSK-3β can enhance the stability of β-catenin, which can bind to TCF/LEF transcription factors in the nucleus and modulate BMP-1 gene expression. | ||||||
GSK-3 Inhibitor IX | 667463-62-9 | sc-202634 sc-202634A sc-202634B | 1 mg 10 mg 50 mg | $58.00 $188.00 $884.00 | 10 | |
BIO is a specific inhibitor of GSK-3β. By inhibiting GSK-3β, it stabilizes β-catenin, which can bind to TCF/LEF transcription factors in the nucleus and modulate BMP-1 gene expression. | ||||||