Bcl-7a Activators
Chemical activators of Bcl-7a initiate a cascade of intracellular events leading to its phosphorylation and subsequent activation. Phorbol 12-myristate 13-acetate (PMA) engages this process by activating protein kinase C (PKC), which directly phosphorylates Bcl-7a. Similarly, diacylglycerol (DAG), a natural activator of PKC, and bryostatin 1, a modulator of PKC, can also promote the activation of Bcl-7a through PKC-mediated phosphorylation. Phosphatidic acid, another lipid second messenger, activates PKC, potentially leading to the phosphorylation of Bcl-7a. Forskolin, by elevating intracellular cAMP levels, activates protein kinase A (PKA) which can then target Bcl-7a. Ionomycin and A23187, both calcium ionophores, raise intracellular calcium levels, which activate calcium-dependent PKC isoforms, thereby facilitating the phosphorylation and activation of Bcl-7a.
In contrast, okadaic acid and calyculin A, inhibitors of protein phosphatases 1 and 2A, prevent the dephosphorylation of proteins, indirectly maintaining Bcl-7a in a phosphorylated state. Thapsigargin, by inhibiting the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), increases cytosolic calcium concentration, which can activate calcium-dependent kinases that phosphorylate Bcl-7a. FTY720, after being phosphorylated to form FTY720-P, acts on sphingosine-1-phosphate receptors, which can initiate signaling pathways leading to the activation of kinases that phosphorylate Bcl-7a. Anisomycin, while primarily known as a protein synthesis inhibitor, can activate stress-activated protein kinases (SAPKs) like JNK, which may then target Bcl-7a for phosphorylation, resulting in its activation. Each of these chemicals, through their distinct mechanisms, ensures the phosphorylation and activation of Bcl-7a, thereby influencing its role in cellular signaling pathways.
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Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which in turn can phosphorylate Bcl-7a, leading to its activation. PKC-mediated phosphorylation is a common mechanism for the functional activation of proteins involved in signal transduction pathways. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits protein phosphatases 1 and 2A, leading to increased phosphorylation levels of many proteins. This could facilitate the phosphorylation and consequent activation of Bcl-7a by kinases by preventing its dephosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Similar to okadaic acid, Calyculin A is an inhibitor of protein phosphatases 1 and 2A. By inhibiting dephosphorylation, it can enhance the phosphorylation state of Bcl-7a, leading to its activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calcium-dependent kinases such as PKC. This could lead to the phosphorylation and activation of Bcl-7a. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to increased cytosolic calcium levels, which may activate calcium-dependent kinases that phosphorylate and activate Bcl-7a. | ||||||
Phosphatidic Acid, Dipalmitoyl | 169051-60-9 | sc-201057 sc-201057B sc-201057A | 100 mg 250 mg 500 mg | $104.00 $239.00 $409.00 | ||
Phosphatidic acid can serve as a lipid second messenger, activating PKC which could then phosphorylate and activate Bcl-7a. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $240.00 | 9 | |
Bryostatin 1 acts as a PKC modulator. While it can function as both an activator and inhibitor, in the context of PKC activation, it may indirectly lead to the phosphorylation and subsequent activation of Bcl-7a. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $46.00 $249.00 | 2 | |
Diacylglycerol activates PKC, which subsequently might phosphorylate Bcl-7a, leading to its functional activation within the cell. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is a calcium ionophore that can increase intracellular calcium concentration, thereby potentially activating calcium-dependent kinases which may phosphorylate and activate Bcl-7a. | ||||||
FTY720 | 162359-56-0 | sc-202161 sc-202161A sc-202161B | 1 mg 5 mg 25 mg | $32.00 $75.00 $118.00 | 14 | |
FTY720 can be phosphorylated to form FTY720-P, which acts as a sphingosine-1-phosphate receptor modulator, possibly leading to downstream signaling events that include activation of kinases that could phosphorylate and activate Bcl-7a. | ||||||