BC034069 Activators
Glucokinase (GCK) Activators are a diverse set of chemical compounds that enhance the functional activity of GCK through various biochemical pathways. Glucose, the primary physiological substrate of GCK, directly increases the enzyme's activity by improving its kinetic properties and inducing a favorable conformational state for catalysis. Similar direct activation is achieved allosterically by fructose-1-phosphate, which binds to a regulatory site on GCK, augmenting its affinity for glucose. Manoheptulose and sorbitol also modulate GCK activity, with the former stabilizing the enzyme in a high-affinity state at low concentrations, and the latter being converted to fructose, subsequently enhancing GCK activity through the production of fructose-1-phosphate. Glucosamine and glyceraldehyde further contribute to GCK activation by generating allosteric effectors that mimic glucose-6-phosphate, reinforcing the enzyme's active conformation.
BC034069 activators are a class of compounds that can amplify the activity of the BC034069 protein. Initial discovery of these activators is typically driven by comprehensive high-throughput screening (HTS) methodologies. This pivotal phase employs diverse chemical libraries, where thousands of molecules are assayed for their ability to modulate the activity of BC034069. The assays are designed to quantitatively assess changes in BC034069 function, often using detectable signals such as fluorescence or luminescence that increase in response to activation. Compounds that demonstrate a capacity to upregulate BC034069 activity are singled out for further examination. These primary hits are then subjected to a battery of secondary assays that are more focused on the BC034069 protein, eliminating false positives and ensuring the specificity of the activation effect.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is a potent activator of protein kinase C (PKC), which phosphorylates target proteins. If BC034069 is a substrate for PKC, its phosphorylation state and functional activity could be directly increased through this pathway. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin acts as a calcium ionophore, increasing intracellular calcium levels. Elevated calcium may activate calcium-dependent kinases such as calmodulin-dependent kinase (CaMK), which might directly phosphorylate and activate BC034069. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, which increases cAMP and cGMP levels in cells by preventing their degradation. This rise in cyclic nucleotides may stimulate kinases like PKA or PKG, potentially enhancing the activity of BC034069 through phosphorylation. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is an ionophore that facilitates the influx of calcium ions into the cytoplasm. Increased intracellular calcium can activate various calcium-sensitive enzymes that might influence BC034069 activation. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is a polyphenol that can inhibit various protein kinases. If BC034069 functions are regulated by one of these kinases, EGCG could enhance BC034069's activity by reducing inhibitory phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can also activate stress-activated protein kinases (SAPKs/JNKs). If BC034069 is activated by these kinases, anisomycin could indirectly enhance BC034069's activity through this stress pathway. | ||||||
BAY 11-7082 | 19542-67-7 | sc-200615B sc-200615 sc-200615A | 5 mg 10 mg 50 mg | $61.00 $83.00 $349.00 | 155 | |
BAY 11-7082 irreversibly inhibits NF-κB activation by blocking the phosphorylation of IκBα. If BC034069 is negatively regulated by NF-κB, inhibition of NF-κB could result in the enhancement of BC034069's activity. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a specific inhibitor of phosphoinositide 3-kinases (PI3K). If BC034069 is activated in a PI3K/Akt pathway-dependent manner, inhibition of this pathway could reduce negative feedback, potentially enhancing BC034069's activity. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $88.00 $342.00 | 284 | |
SB203580 is a specific inhibitor of p38 MAP kinase. If BC034069's activity is upregulated by stress or inflammatory signals that are negatively regulated by p38, inhibition by SB203580 could enhance BC034069's activity. | ||||||
U-0126 | 109511-58-2 | sc-222395 sc-222395A | 1 mg 5 mg | $63.00 $241.00 | 136 | |
U0126 is a selective inhibitor of mitogen-activated protein kinase kinase (MEK), which is part of the MAPK/ERK pathway. If BC034069 is regulated through this pathway, U0126 could lead to the upregulation of BC034069's activity by modulating the pathway. | ||||||