ASPDH Activators
ASPDH can influence the protein's activity through various mechanisms, primarily by modulating the phosphorylation state which is critical for its function. Bisindolylmaleimide I, for example, is a selective inhibitor of protein kinase C, but it can also activate other kinases. This dual role allows it to enhance the phosphorylation of ASPDH, thereby increasing its activity. Forskolin operates through a different mechanism by directly stimulating adenylyl cyclase, subsequently raising cAMP levels which activate protein kinase A (PKA). PKA then phosphorylates ASPDH, enhancing its activity. Similarly, PMA activates PKC, possibly leading to a direct or indirect increase in ASPDH phosphorylation. Ionomycin raises intracellular calcium levels, paving the way for calmodulin-dependent kinases to phosphorylate and activate ASPDH.
Other chemicals work by maintaining ASPDH in a phosphorylated state. Okadaic Acid and Calyculin A, for instance, inhibit protein phosphatases PP1 and PP2A, which normally dephosphorylate proteins, thus keeping ASPDH active. Anisomycin activates stress-activated protein kinases that can phosphorylate ASPDH. In addition, compounds like Epigallocatechin gallate (EGCG) modulate kinase activity, possibly enhancing the phosphorylation and activity of ASPDH. Dibutyryl-cAMP, a cAMP analog, diffuses into cells to activate PKA, which fosters further phosphorylation of ASPDH. The PI3K inhibitor LY294002 might activate compensatory pathways that ultimately lead to ASPDH activation. Similarly, SB203580 and PD98059, known inhibitors of p38 MAPK and MEK respectively, can result in the activation of alternative kinases that may phosphorylate and thus activate ASPDH. These chemical activators collectively ensure that ASPDH is primed for its enzymatic role through a diverse array of signaling pathways.
SEE ALSO...
Items 1 to 10 of 12 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $103.00 $237.00 | 36 | |
Bisindolylmaleimide I serves as a potent and selective inhibitor of protein kinase C (PKC), but it also activates other kinases upon which ASPDH potentially relies for its activation state. By activating these kinases, Bisindolylmaleimide I would enhance the phosphorylation status of ASPDH, promoting its activity. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, increasing the levels of cAMP, a secondary messenger that activates PKA. PKA could then phosphorylate ASPDH, leading to an increase in its enzymatic activity. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is a diacylglycerol analog that activates PKC. PKC can phosphorylate ASPDH directly or indirectly, leading to an increase in its enzymatic activity. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular Ca2+ concentration, which can activate calmodulin-dependent kinase (CaMK), which in turn may phosphorylate and activate ASPDH. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A), which prevents dephosphorylation of proteins. This indirectly maintains ASPDH in a phosphorylated and active state. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000C | 10 µg 100 µg 1 mg | $160.00 $750.00 $3000.00 | 59 | |
Similar to okadaic acid, Calyculin A is an inhibitor of PP1 and PP2A, leading to increased phosphorylation and sustained activation of ASPDH. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a potent activator of the stress-activated protein kinases (SAPKs), which can phosphorylate ASPDH, resulting in the activation of its enzymatic functions. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG has been shown to have kinase-modulating properties and could enhance the activity of kinases that phosphorylate and activate ASPDH. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cAMP analog that penetrates cell membranes and activates PKA intracellularly, which can then phosphorylate and activate ASPDH. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a PI3K inhibitor, but by inhibiting this kinase, it can result in the activation of compensatory pathways that lead to the activation of ASPDH through feedback mechanisms. | ||||||