ALKBH5 Activators
ALKBH5 activators fall within a unique class of chemicals that interact with the AlkB homolog 5 (ALKBH5) protein, which is an enzyme from the AlkB family of dioxygenases. The ALKBH proteins, in general, are associated with the process of oxidative demethylation, a vital biochemical reaction involving the removal of methyl groups from various substrates, including nucleic acids. ALKBH5, in particular, is known to target and demethylate N6-methyladenosine (m6A) in RNA. This demethylation process is crucial as it affects the metabolism and functionality of RNA within cells. ALKBH5 activators, therefore, are compounds that enhance the enzymatic activity of ALKBH5, increasing the rate at which it demethylates m6A sites in RNA. This can have a significant impact on the RNA's secondary structure, stability, and its interaction with RNA-binding proteins, subsequently affecting the regulation of gene expression.
From a biochemical perspective, ALKBH5 activators can be diverse in their structure and mode of action. Typically, they may bind to the ALKBH5 enzyme, causing conformational changes that result in an increased affinity of the enzyme for its RNA substrate or an increased catalytic activity. The exact mechanism of activation, however, can vary depending on the specific chemical structure of the activator. The interaction between an activator and ALKBH5 can be direct, influencing the active site of the enzyme, or it might be allosteric, impacting the enzyme activity without directly interfering with the active site. Moreover, these activators might also influence the expression levels of ALKBH5 or its availability in the cellular environment by affecting the enzymes' stability or the transcriptional and translational mechanisms that govern its synthesis.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
5-Aza-2′-Deoxycytidine | 2353-33-5 | sc-202424 sc-202424A sc-202424B | 25 mg 100 mg 250 mg | $214.00 $316.00 $418.00 | 7 | |
A DNA methyltransferase inhibitor, it can modify DNA methylation patterns which may subsequently affect RNA synthesis rates or types. Changes in RNA abundance or structure can promote increased interaction with ALKBH5, hence its potential activation. | ||||||
A 83-01 | 909910-43-6 | sc-203791 sc-203791A | 10 mg 50 mg | $198.00 $650.00 | 16 | |
By inhibiting TGF-β signaling, this compound can modify cellular transcriptional profiles. Altered RNA outputs may favor ALKBH5 binding and activation due to shifts in m^6A-laden RNA substrates. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Elevates cAMP levels. Increased cAMP can stimulate RNA processing and transcription changes. The resultant modified RNA landscape can present more substrates for ALKBH5 demethylation, potentially activating it. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PKC activator. By influencing RNA processing through PKC signaling, this compound may enhance RNA substrates that are suitable for ALKBH5 demethylation, promoting its activation. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
A PI3K inhibitor, it impacts the PI3K/Akt pathway which is linked to transcription regulation. Shifts in transcriptional activity can lead to an altered RNA landscape, thereby influencing ALKBH5's demethylating capacity and possibly its activation. | ||||||
Resveratrol | 501-36-0 | sc-200808 sc-200808A sc-200808B | 100 mg 500 mg 5 g | $60.00 $185.00 $365.00 | 64 | |
By activating sirtuins, cellular RNA metabolism can be impacted. The resulting change in RNA populations can lead to a higher prevalence of substrates for ALKBH5, leading to its increased activation. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
By inhibiting the mTOR pathway, transcription and RNA processing may be affected. The modified RNA pool can lead to increased ALKBH5 substrate availability, thereby potentially activating it. | ||||||
(±)-JQ1 | 1268524-69-1 | sc-472932 sc-472932A | 5 mg 25 mg | $226.00 $846.00 | 1 | |
As a BET bromodomain inhibitor, JQ1 affects transcriptional regulation. Changes in RNA synthesis rates or types can alter the substrate availability for ALKBH5, thereby modulating its activation. | ||||||
BIX01294 hydrochloride | 1392399-03-9 | sc-293525 sc-293525A sc-293525B | 1 mg 5 mg 25 mg | $36.00 $110.00 $400.00 | ||
As a G9a inhibitor, it affects chromatin state and transcription. This can alter RNA types or levels in the cell, potentially providing more substrates for ALKBH5, hence modulating its activation. | ||||||
RG 108 | 48208-26-0 | sc-204235 sc-204235A | 10 mg 50 mg | $128.00 $505.00 | 2 | |
By inhibiting DNA methyltransferases and affecting DNA methylation, RNA synthesis can be affected. The altered RNA landscape can influence ALKBH5's activity by presenting more substrates for demethylation. | ||||||