4930525F21Rik Activators
Chemical activators of FAM227B can initiate a cascade of events within cells that lead to the protein's activation. Forskolin operates by directly stimulating adenylate cyclase, which catalyzes the conversion of ATP to cyclic AMP (cAMP). An increase in cAMP levels triggers the activation of protein kinase A (PKA), a crucial enzyme that can then phosphorylate and activate FAM227B. Similarly, IBMX and Rolipram, as inhibitors of phosphodiesterases, prevent the degradation of cAMP, thereby sustaining the activation state of PKA which, in turn, acts on FAM227B. The PDE4 inhibitor shares a comparable mechanism, albeit with a more targeted inhibition of the phosphodiesterase 4 subtype, resulting in increased cAMP levels and subsequent PKA-mediated FAM227B activation.
Moreover, adrenergic compounds like Epinephrine and Isoproterenol also lead to heightened cAMP levels by stimulating adrenergic receptors that activate adenylate cyclase. The ensuing PKA activation can phosphorylate FAM227B. Anisomycin, although primarily known as a protein synthesis inhibitor, can activate stress-activated protein kinases that may also target and phosphorylate FAM227B. Additionally, PMA, through the activation of protein kinase C (PKC), and Ionomycin, by increasing intracellular calcium and subsequently activating PKC, can initiate phosphorylation events leading to FAM227B activation. Bay K8644's selective activation of L-type calcium channels further contributes to the increase in intracellular calcium, which activates calcium-dependent kinases such as PKC, again leading to the activation of FAM227B. Dibutyryl-cAMP, a cAMP analog, bypasses cellular receptors and directly activates cAMP-dependent PKA, promoting the phosphorylation of FAM227B. Lastly, Zaprinast inhibits PDE5, which results in an accumulation of cGMP and activates PKG, a kinase that can also phosphorylate and activate FAM227B.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
Non-selective inhibitor of phosphodiesterases, which prevents the breakdown of cAMP and cGMP, potentially sustaining the activation state of PKA and PKG pathways, which may phosphorylate and activate FAM227B. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $41.00 $104.00 $201.00 $1774.00 $16500.00 | ||
Stimulates adrenergic receptors leading to activation of adenylate cyclase and an increase in cAMP levels, thereby activating PKA, which could phosphorylate and activate FAM227B. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
A synthetic catecholamine that acts as a non-selective beta-adrenergic agonist, leading to activation of adenylate cyclase, which increases cAMP and thereby activates PKA, potentially leading to phosphorylation and activation of FAM227B. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
A selective inhibitor of PDE4, enhancing intracellular cAMP accumulation, which activates PKA, and this activated PKA may target and activate FAM227B through phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Inhibits protein synthesis at the level of peptidyl transferase which can lead to the activation of stress-activated protein kinases/JNK pathways, and these kinases can phosphorylate and activate a variety of substrates, potentially including FAM227B. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Activates protein kinase C (PKC), which can lead to the activation of downstream kinases and transcription factors that may phosphorylate and activate FAM227B. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Increases intracellular calcium concentration, which can activate calcium-dependent protein kinases such as PKC, which might subsequently phosphorylate and activate FAM227B. | ||||||
(±)-Bay K 8644 | 71145-03-4 | sc-203324 sc-203324A sc-203324B | 1 mg 5 mg 50 mg | $84.00 $196.00 $817.00 | ||
Selectively activates L-type calcium channels, leading to an increased intracellular calcium concentration which activates calcium-dependent proteins such as PKC, which can then phosphorylate and activate FAM227B. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
A cell-permeable cAMP analog that directly activates cAMP-dependent pathways such as PKA, leading to potential phosphorylation and activation of FAM227B. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $105.00 $250.00 | 8 | |
Inhibits PDE5 leading to increased cGMP levels, which can enhance PKG activity, and PKG can phosphorylate and activate proteins that may include FAM227B in its range of substrates. | ||||||