ZAP-70 Antibody (G-4): sc-17760
- ZAP-70 Antibody (G-4) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 253-304 of ZAP-70 of human origin
- recommended for detection of ZAP-70 of human origin by WB, IP, IF, FCM and ELISA
- available conjugated to phycoerythrin for IF, IHC(P) and FCM
- See ZAP-70 (1E7.2): sc-32760 for ZAP-70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZAP-70 Antibody (G-4) for WB applications. These reagents are now offered in bundles with ZAP-70 Antibody (G-4) (see ordering information below).
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ZAP-70 Antibody (G-4) is a mouse monoclonal IgG1 light chain antibody that detects ZAP-70 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm of T cells, ZAP-70 is recruited to the T cell receptor complex upon antigen recognition, where ZAP-70 becomes phosphorylated and activates downstream signaling pathways that lead to T cell proliferation and differentiation. The importance of ZAP-70 in immune function is underscored by ZAP-70′s association with severe combined immunodeficiency when mutated, highlighting ZAP-70′s critical role in maintaining effective immune responses. ZAP-70 (G-4) antibody is an invaluable tool for researchers studying T cell signaling and implications in immunological disorders, providing insights into the mechanisms that govern T cell activation and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZAP-70 Antibody (G-4) References:
- ZAP-70 in B cell malignancies. | Orchard, J., et al. 2005. Leuk Lymphoma. 46: 1689-98. PMID: 16263570
- Detection methods of ZAP-70 in chronic lymphocytic leukemia. | Wang, YH., et al. 2012. Clin Exp Med. 12: 69-77. PMID: 21691729
- Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia. | Vroblova, V., et al. 2012. Hematology. 17: 268-74. PMID: 22971532
- ZAP-70 in Signaling, Biology, and Disease. | Au-Yeung, BB., et al. 2018. Annu Rev Immunol. 36: 127-156. PMID: 29237129
- ZAP-70 in chronic lymphocytic leukemia: A meta-analysis. | Liu, Y., et al. 2018. Clin Chim Acta. 483: 82-88. PMID: 29680229
- ZAP-70 Shapes the Immune Microenvironment in B Cell Malignancies. | Chen, J., et al. 2020. Front Oncol. 10: 595832. PMID: 33194762
- Discovery of a potent, selective, and covalent ZAP-70 kinase inhibitor. | Rao, D., et al. 2021. Eur J Med Chem. 219: 113393. PMID: 33845236
- SYK and ZAP70 kinases in autoimmunity and lymphoid malignancies. | Leveille, E., et al. 2022. Cell Signal. 94: 110331. PMID: 35398488
- Discovery and Structural Optimization of Covalent ZAP-70 Kinase Inhibitors against Psoriasis. | Rao, D., et al. 2023. J Med Chem. 66: 12018-12032. PMID: 37594408
- ZAP-70 augments tonic B-cell receptor and CCR7 signaling in IGHV-unmutated chronic lymphocytic leukemia. | Chen, J., et al. 2024. Blood Adv. 8: 1167-1178. PMID: 38113463
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAP-70 Antibody (G-4) | sc-17760 | 200 µg/ml | $322.00 | |||
ZAP-70 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-536584 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZAP-70 Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-533735 | 200 µg Ab; 40 µg BP | $361.00 | |||
ZAP-70 Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-544692 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZAP-70 Antibody (G-4) PE | sc-17760 PE | 200 µg/ml | $349.00 |