ZAP-70 Antibody (1E7.2): sc-32760
- ZAP-70 Antibody (1E7.2) is a mouse monoclonal IgG1 κ ZAP-70 antibody, cited in 31 publications, provided at 200 µg/ml
- raised against amino acids 282-307 of ZAP-70 of human origin
- ZAP-70 Antibody (1E7.2) is recommended for detection of ZAP-70 of mouse, rat and human origin by WB, IP, IF and FCM
- Anti-ZAP-70 Antibody (1E7.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZAP-70 Antibody (1E7.2) for WB applications. These reagents are now offered in bundles with ZAP-70 Antibody (1E7.2) (see ordering information below).
QUICK LINKS
SEE ALSO...
ZAP-70 Antibody (1E7.2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZAP-70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-ZAP-70 antibody (1E7.2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZAP-70 plays a crucial role in T cell signaling, particularly in the activation of T lymphocytes following antigen recognition by the T cell receptor (TCR). Upon TCR engagement, ZAP-70 is recruited to the receptor complex, where phosphorylation activates downstream signaling pathways that lead to T cell activation, proliferation, and differentiation. This process is vital for the adaptive immune response, enabling T cells to effectively respond to pathogens and maintain immune homeostasis. ZAP-70′s importance is underscored by its association with severe immunodeficiency when mutated, highlighting an essential role in T cell function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZAP-70 Antibody (1E7.2) References:
- ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain. | Chan, AC., et al. 1992. Cell. 71: 649-62. PMID: 1423621
- ZAP-70 expression is low in normal precursor B cells or hematogones. | Davis, BH. and Schwartz, M. 2006. Cytometry B Clin Cytom. 70: 315-9. PMID: 16906572
- The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein. | Chan, AC., et al. 1991. Proc Natl Acad Sci U S A. 88: 9166-70. PMID: 1717999
- CD3 eta and CD3 zeta are alternatively spliced products of a common genetic locus and are transcriptionally and/or post-transcriptionally regulated during T-cell development. | Clayton, LK., et al. 1991. Proc Natl Acad Sci U S A. 88: 5202-6. PMID: 1828894
- The structure, regulation, and function of ZAP-70. | Au-Yeung, BB., et al. 2009. Immunol Rev. 228: 41-57. PMID: 19290920
- The structure and signalling functions of the invariant T cell receptor components. | Frank, SJ., et al. 1990. Semin Immunol. 2: 89-97. PMID: 2151801
- The isolation and characterization of the murine T cell antigen receptor zeta chain gene. | Baniyash, M., et al. 1989. J Biol Chem. 264: 13252-7. PMID: 2787796
- The T cell antigen receptor zeta chain is tyrosine phosphorylated upon activation. | Baniyash, M., et al. 1988. J Biol Chem. 263: 18225-30. PMID: 3142873
- Immunomodulatory drugs activate NK cells via both Zap-70 and cereblon-dependent pathways. | Hideshima, T., et al. 2021. Leukemia. 35: 177-188. PMID: 32238854
- The T cell receptor/CD3 complex: a dynamic protein ensemble. | Clevers, H., et al. 1988. Annu Rev Immunol. 6: 629-62. PMID: 3289580
- Disulfide linkage of the zeta and eta chains of the T cell receptor. Possible identification of two structural classes of receptors. | Baniyash, M., et al. 1988. J Biol Chem. 263: 9874-8. PMID: 3290215
- Zeta phosphorylation without ZAP-70 activation induced by TCR antagonists or partial agonists. | Madrenas, J., et al. 1995. Science. 267: 515-8. PMID: 7824949
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAP-70 Antibody (1E7.2) | sc-32760 | 200 µg/ml | $316.00 | |||
ZAP-70 Antibody (1E7.2): m-IgG Fc BP-HRP Bundle | sc-528467 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZAP-70 Antibody (1E7.2): m-IgGκ BP-HRP Bundle | sc-520855 | 200 µg Ab, 40 µg BP | $354.00 | |||
ZAP-70 Antibody (1E7.2): m-IgG1 BP-HRP Bundle | sc-542946 | 200 µg Ab; 20 µg BP | $354.00 | |||
ZAP-70 Antibody (1E7.2) AC | sc-32760 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZAP-70 Antibody (1E7.2) HRP | sc-32760 HRP | 200 µg/ml | $316.00 | |||
ZAP-70 Antibody (1E7.2) FITC | sc-32760 FITC | 200 µg/ml | $330.00 | |||
ZAP-70 Antibody (1E7.2) PE | sc-32760 PE | 200 µg/ml | $343.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 488 | sc-32760 AF488 | 200 µg/ml | $357.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 546 | sc-32760 AF546 | 200 µg/ml | $357.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 594 | sc-32760 AF594 | 200 µg/ml | $357.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 647 | sc-32760 AF647 | 200 µg/ml | $357.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 680 | sc-32760 AF680 | 200 µg/ml | $357.00 | |||
ZAP-70 Antibody (1E7.2) Alexa Fluor® 790 | sc-32760 AF790 | 200 µg/ml | $357.00 |