Topo IIα Antibody (G-9): sc-166232

Topo IIα Antibody (G-9) is a mouse monoclonal IgG1 antibody engineered to detect DNA topoisomerase II alpha (Topo IIα), also known as Top2α. Topo IIα monoclonal antibody (G-9) targets an epitope spanning amino acids 1301-1531 of the human Topo IIα protein, ensuring precise binding and consistent performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA topoisomerase II alpha is a critical nuclear enzyme responsible for managing the topological state of DNA, which is essential for processes like DNA replication, transcription, and chromosome segregation. Located predominantly in the cell nucleus, Topo IIα facilitates the resolution of DNA supercoiling and tangling by introducing transient double-strand breaks and subsequently rejoining the DNA strands, thereby maintaining genomic stability and ensuring accurate cell division. The nuclear localization of Topo IIα underscores its pivotal role in cell proliferation and its importance as a target for various anticancer therapies, making Topo IIα a key focus in cancer research and treatment development. Topo IIα exists alongside its isoform Topo IIβ, with each isoform exhibiting distinct cellular functions and tissue distribution patterns, highlighting the specificity of Topo IIα (G-9) antibody for the alpha variant. The robust reactivity of Topo IIα (G-9) monoclonal antibody with human Topo IIα makes Topo IIα (G-9) antibody an indispensable tool for researchers studying DNA topology, cell cycle regulation, and the mechanisms underlying topoisomerase-targeting drugs.

The preferred Topo IIα antibody is Topo IIα Antibody (F-12): sc-365916.