Topo IIα Antibody (E-10): sc-365799
- Topo IIα Antibody (E-10) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1511-1530 at the C-terminus of Topo IIα of human origin
- recommended for detection of Topo IIα of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Topo IIα Antibody (E-10) for WB applications. This reagent is now offered in a bundle with Topo IIα Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Topo IIα Antibody (E-10) is a mouse monoclonal IgM kappa light chain antibody designed to detect DNA topoisomerase II alpha (Topo IIα), also known as Top2α, a vital enzyme involved in the regulation of DNA topology. Anti-Topo IIα antibody (E-10) specifically targets an epitope located between amino acids 1511-1530 at the C-terminus of human Topo IIα, ensuring precise binding and reliable performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Topo IIα is a nuclear enzyme that plays a critical role in essential cellular processes including DNA replication, transcription, chromosome condensation, and segregation by introducing transient double-strand breaks and rejoining DNA strands, thereby managing DNA supercoiling and maintaining genomic stability. The structure of Topo IIα comprises distinct domains, including the ATPase domain, DNA-binding core, and a C-terminal regulatory domain, which is subject to various post-translational modifications such as phosphorylation and sumoylation. These modifications are crucial for regulating Topo IIα′s enzymatic activity, subcellular localization, and interactions with other proteins, thereby ensuring proper cell cycle progression and response to cellular stress. Topo IIα monoclonal antibody (E-10)′s reactivity extends to Topo IIα from multiple species, including mouse, rat, human, and porcine, making this antibody a versatile tool for diverse research applications. Additionally, Topo IIα exists alongside its isoform Topo IIβ, each exhibiting unique structural and functional characteristics, with Topo IIα primarily associated with cell proliferation and cancer progression. By facilitating the study of Topo IIα′s post-translational modifications and their effects on function, Topo IIα (E-10) antibody is an invaluable resource for researchers investigating DNA topology, cell cycle regulation, and the mechanisms of topoisomerase-targeting therapeutics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo IIα Antibody (E-10) | sc-365799 | 200 µg/ml | $316.00 | |||
Topo IIα Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-550747 | 200 µg Ab; 40 µg BP | $354.00 | |||
Topo IIα (E-10) Neutralizing Peptide | sc-365799 P | 100 µg/0.5 ml | $68.00 |