Topo IIα Antibody (A-8): sc-165986

Topo IIα Antibody (A-8) is a mouse monoclonal IgG1 antibody designed to detect DNA topoisomerase II alpha (Topo IIα). Anti-Topo IIα antibody (A-8) targets an epitope within amino acids 1301-1531 of the human Topo IIα protein, ensuring precise binding and reliable performance across various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA topoisomerase II alpha is an essential nuclear enzyme that plays a critical role in regulating the topological state of DNA, facilitating vital cellular processes including DNA replication, transcription, and chromosome segregation. By introducing transient double-strand breaks and subsequently rejoining DNA strands, Topo IIα alleviates DNA supercoiling and tangling, thereby maintaining genomic stability and ensuring proper cell division. The functionality of Topo IIα is particularly important in cell proliferation and has been identified as a key target for numerous anticancer drugs, underscoring its significance in cancer research and therapy. Topo IIα exists as one of two isoforms, with Topo IIβ being the other, each exhibiting distinct cellular functions and tissue distribution patterns. The robust reactivity of Topo IIα (A-8) monoclonal antibody with human Topo IIα makes this reagent an invaluable tool for researchers investigating DNA topology, cell cycle regulation, and the mechanisms of action of topoisomerase-targeting therapeutics. Anti-Topo IIα antibody (A-8) advances our understanding of this pivotal enzyme′s role in maintaining cellular integrity and its implications in various diseases.

The preferred Topo IIα antibody is Topo IIα Antibody (F-12): sc-365916.