Thrombin R Antibody (ATAP2): sc-13503

Thrombin R Antibody (ATAP2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Thrombin R protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Thrombin R monoclonal antibody (ATAP2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thrombin R, also known as protease-activated receptor-1 (PAR-1), plays a crucial role in the regulation of hemostasis and inflammation by mediating the effects of thrombin, a serine protease that is pivotal in platelet aggregation and blood coagulation. Upon activation by thrombin, Thrombin R undergoes cleavage, releasing a 41 amino acid peptide that acts as a potent platelet agonist, leading to an increase in cytosolic calcium concentration. This activation is essential for the physiological response to vascular injury, as it promotes platelet activation and aggregation, thereby facilitating clot formation. Thrombin R protein is predominantly located on the cell surface, where it can interact with thrombin, but also cycles between the cell surface and an intracellular pool, which is vital for maintaining cellular responsiveness to thrombin. Additionally, human Thrombin R is regulated by transcription factors such as Sp1 and Sp3, highlighting the complexity of its expression and function in various physiological and pathological contexts.

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Thrombin R Antibody (ATAP2) References:

1. PAR-1 and thrombin: the ties that bind the microenvironment to melanoma metastasis.  |  Zigler, M., et al. 2011. Cancer Res. 71: 6561-6. PMID: 22009534
2. Protease-activated receptor-1 (PAR-1): a promising molecular target for cancer.  |  Liu, X., et al. 2017. Oncotarget. 8: 107334-107345. PMID: 29291033
3. Thrombin Signaling Promotes Pancreatic Adenocarcinoma through PAR-1-Dependent Immune Evasion.  |  Yang, Y., et al. 2019. Cancer Res. 79: 3417-3430. PMID: 31048498
4. Thrombin/PAR-1 activation induces endothelial damages via NLRP1 inflammasome in gestational diabetes.  |  Liu, Y., et al. 2020. Biochem Pharmacol. 175: 113849. PMID: 32059841
5. G protein-coupled receptor kinase 5 regulates thrombin signaling in platelets via PAR-1.  |  Downes, K., et al. 2022. Blood Adv. 6: 2319-2330. PMID: 34581777
6. The USP7-STAT3-granzyme-Par-1 axis regulates allergic inflammation by promoting differentiation of IL-5-producing Th2 cells.  |  Kumagai, J., et al. 2023. Proc Natl Acad Sci U S A. 120: e2302903120. PMID: 38015852
7. Differential transcriptional regulation of the human thrombin receptor gene by the Sp family of transcription factors in human endothelial cells.  |  Wu, Y., et al. 1998. Biochem J. 330 (Pt 3): 1469-74. PMID: 9494121
8. The cleaved peptide of the thrombin receptor is a strong platelet agonist.  |  Furman, MI., et al. 1998. Proc Natl Acad Sci U S A. 95: 3082-7. PMID: 9501219
9. Thrombin.  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
10. Gene and locus structure and chromosomal localization of the protease-activated receptor gene family.  |  Kahn, ML., et al. 1998. J Biol Chem. 273: 23290-6. PMID: 9722561
11. Separate signals for agonist-independent and agonist-triggered trafficking of protease-activated receptor 1.  |  Shapiro, MJ. and Coughlin, SR. 1998. J Biol Chem. 273: 29009-14. PMID: 9786906
12. Analysis of a Ca2+-activated K+ channel that mediates hyperpolarization via the thrombin receptor pathway.  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thrombin R Antibody (ATAP2) has 195 citations in a variety of scientific publications.

Primary antibodies like Anti-Thrombin R Antibody (ATAP2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thrombin R Antibody (ATAP2) Specifications

Alternate Product Name: thrombin receptor

Product Clone Name: ATAP2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 42-55

Epitope Target: Thrombin R

Epitope Species: human

Product Reactivity/Detection: Thrombin R

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 195 citations in various scientific publications

Available Conjugates/Forms for Thrombin R Antibody (ATAP2): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin