Smad5 Antibody (YY-6): sc-101151

Smad5 Antibody (YY-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Smad5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Smad5 antibody (YY-6) is available as the non-conjugated format. Smad5 plays a crucial role in the bone morphogenetic protein (BMP) signaling pathway, which is essential for various biological processes, including embryonic development, tissue homeostasis, and regulation of cell growth and differentiation. Smad5 acts as a transcription factor that transduces signals from BMPs, leading to activation of target genes involved in osteogenesis and chondrogenesis. Proper functioning of Smad5 is vital, as dysregulation of BMP signaling has been implicated in various diseases, including cancer and skeletal disorders. Additionally, Smad5 interacts with other Smad proteins, such as Smad1 and Smad4, to form complexes that facilitate transcriptional regulation of downstream target genes. This interaction underscores Smad5′s importance in maintaining balanced signaling pathways that govern cellular responses to growth factors.

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Smad5 Antibody (YY-6) References:

1. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury.  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
2. Smads and chromatin modulation.  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
3. TGF-beta signalling through the Smad pathway.  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
4. Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis.  |  Monsivais, D., et al. 2021. Nat Commun. 12: 3386. PMID: 34099644
5. Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.  |  Liao, Z., et al. 2024. Elife. 12: PMID: 38536963
6. A human Mad protein acting as a BMP-regulated transcriptional activator.  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
7. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
8. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
9. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
10. Smad6 inhibits signalling by the TGF-beta superfamily.  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
11. Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase.  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
12. TGF-beta signalling from cell membrane to nucleus through SMAD proteins.  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smad5 Antibody (YY-6) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Smad5 Antibody (YY-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smad5 Antibody (YY-6) Specifications

Alternate Product Name: Smad5

Product Clone Name: YY-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 105-182

Epitope Target: Smad5

Epitope Species: human

Product Reactivity/Detection: Smad5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Smad5 Antibody (YY-6): N/A

The preferred Smad1/2/3 antibody is Smad1/2/3 Antibody (H-2): sc-7960.