Smad1/2/3 Antibody (H-2): sc-7960

Smad1/2/3 Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Smad1, Smad2, and Smad3 proteins across multiple species, including mouse, rat, human, and mink. Smad1/2/3 mouse monoclonal antibody (H-2) targets amino acids 1-465 of full-length human Smad1 protein, ensuring comprehensive recognition of these critical signaling molecules. Smad proteins, alternatively known as Mothers against dpp (Mad) in Drosophila, play pivotal roles as downstream effectors in Transforming Growth Factor-beta (TGFβ) and Bone Morphogenetic Protein (BMP) signaling pathways. Smad1 (Mad1 or JV4-1) and Smad5 are integral to BMP2 and BMP4 function, while Smad2 (Mad2 or JV18-1) and Smad3 are essential for mediating TGFβ and activin-induced growth modulation. These pathways are fundamental for numerous cellular processes, including proliferation, differentiation, and apoptosis, making Smad proteins crucial for maintaining cellular homeostasis and development. Smad1/2/3 monoclonal antibody (H-2) is versatile in applications, effectively performing in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). By facilitating Smad-mediated signaling studies, Smad1/2/3 antibody (H-2) supports research into various physiological and pathological conditions, including cancer, fibrosis, and developmental disorders. Understanding regulatory mechanisms of Smad proteins, such as inhibitory roles of Smad6 and Smad7, provides deeper insights into TGFβ/BMP pathway modulation. Available in both non-conjugated and multiple conjugated forms, including HRP, PE, FITC, and Alexa Fluor® variants, Smad1/2/3 monoclonal antibody (H-2) is useful for researchers exploring complexities of cellular signaling and gene expression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smad1/2/3 Antibody (H-2) has 78 citations in a variety of scientific publications.

Primary antibodies like Anti-Smad1/2/3 Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smad1/2/3 Antibody (H-2) Specifications

Alternate Product Name: Smad1/2/3

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-465

Epitope Target: Smad1

Epitope Species: human

Product Reactivity/Detection: Smad1, Smad2, Smad3

Species Reactivity/Detection: mouse, rat, human, mink

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 78 citations in various scientific publications

Available Conjugates/Forms for Smad1/2/3 Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Smad3 antibody is Smad3 Antibody (38-Q): sc-101154.