Smad2/3 Antibody (C-8): sc-133098
- Smad2/3 Antibody (C-8) is a mouse monoclonal IgG2a κ, cited in 126 publications, provided at 200 µg/ml
- raised against amino acids 1-425 representing full length Smad3 of human origin
- Anti-Smad2/3 Antibody (C-8) is recommended for detection of Smad2 and Smad3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-Smad2/3 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-133098 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Smad2/3 (C-8): sc-133098.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Smad2/3 Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Smad2/3 Antibody (C-8) (see ordering information below).
Smad2/3 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect both Smad2 and Smad3 proteins across multiple species, including mouse, rat, human, canine, and porcine. anti-Smad2/3 antibody (C-8) targets amino acids 1-425 of the full-length human Smad3 protein, ensuring accurate recognition of these essential signaling molecules. Smad2 and Smad3, also known as Madr2 and Smad3 respectively, are critical mediators of the Transforming Growth Factor-beta (TGFβ) signaling pathway. Structurally, these proteins contain conserved MH1 and MH2 domains that facilitate their role in transmitting signals from cell membrane to nucleus, where they regulate transcription of target genes involved in cell proliferation, differentiation, and apoptosis. The integrity of Smad2/3 is vital for forming complexes with Smad4 and other Smad family members, enabling precise control of various cellular functions and maintaining cellular homeostasis. Smad2/3 monoclonal antibody (C-8) is versatile in applications, performing effectively in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). By facilitating in-depth studies of Smad2 and Smad3-mediated signaling pathways, Smad2/3 (C-8) antibody supports research into numerous physiological and pathological conditions, including cancer, fibrosis, and developmental disorders. Additionally, understanding the structural and functional dynamics of Smad proteins with anti-Smad2/3 antibody (C-8) provides valuable insights into the modulation of TGFβ signaling. Available in both non-conjugated and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® variants, Smad2/3 (C-8) monoclonal antibody is an indispensable tool for researchers exploring the complexities of cellular signaling and gene regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad2/3 Antibody (C-8) | sc-133098 | 200 µg/ml | $316.00 | |||
Smad2/3 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-528750 | 200 µg Ab; 10 µg BP | $354.00 | |||
Smad2/3 Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-521235 | 200 µg Ab, 40 µg BP | $354.00 | |||
Smad2/3 Antibody (C-8) X | sc-133098 X | 200 µg/0.1 ml | $316.00 | |||
Smad2/3 Antibody (C-8) AC | sc-133098 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Smad2/3 Antibody (C-8) HRP | sc-133098 HRP | 200 µg/ml | $316.00 | |||
Smad2/3 Antibody (C-8) FITC | sc-133098 FITC | 200 µg/ml | $330.00 | |||
Smad2/3 Antibody (C-8) PE | sc-133098 PE | 200 µg/ml | $343.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 488 | sc-133098 AF488 | 200 µg/ml | $357.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 546 | sc-133098 AF546 | 200 µg/ml | $357.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 594 | sc-133098 AF594 | 200 µg/ml | $357.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 647 | sc-133098 AF647 | 200 µg/ml | $357.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 680 | sc-133098 AF680 | 200 µg/ml | $357.00 | |||
Smad2/3 Antibody (C-8) Alexa Fluor® 790 | sc-133098 AF790 | 200 µg/ml | $357.00 |