SIRT1 Antibody (B-10): sc-74504

SIRT1 Antibody (B-10) is a mouse monoclonal IgG2b kappa light chain antibody engineered to target amino acids 448-747 of the human SIRT1 protein, facilitating the detection of SIRT1 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. SIRT1, also referred to as SIR2, SIR2L1, and SIR2alpha, is a crucial member of the sirtuin family. SIRT1 possesses a conserved catalytic core composed of a large Rossmann-fold domain and a smaller zinc-binding domain, interconnected by a flexible linker region that is essential for NAD⁺-dependent deacetylase activity. This structural configuration allows SIRT1 to interact with a variety of substrates, including histones and transcription factors, thereby regulating key cellular processes such as gene expression, DNA repair, metabolism, and aging. The precise structure of SIRT1 is vital for function, as this determines substrate specificity, enzymatic efficiency, and interactions with regulatory proteins, making SIRT1 a pivotal target for studies investigating age-related diseases, cancer, and metabolic disorders. Anti-SIRT1 antibody (B-10) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options for diverse experimental setups. By enabling detailed structural and functional analysis, SIRT1 monoclonal antibody (B-10) serves as an indispensable tool for researchers aiming to unravel the complexities of SIRT1′s role in cellular physiology and pathology, ultimately contributing to the advancement of therapeutic strategies targeting this vital protein.

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SIRT1 Antibody (B-10) References:

1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.  |  Frye, RA. 1999. Biochem Biophys Res Commun. 260: 273-9. PMID: 10381378
2. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae SIR2.  |  Afshar, G. and Murnane, JP. 1999. Gene. 234: 161-8. PMID: 10393250
3. The conserved core of a human SIR2 homologue functions in yeast silencing.  |  Sherman, JM., et al. 1999. Mol Biol Cell. 10: 3045-59. PMID: 10473645
4. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.  |  Frye, RA. 2000. Biochem Biophys Res Commun. 273: 793-8. PMID: 10873683
5. Sound silencing: the Sir2 protein and cellular senescence.  |  Defossez, PA., et al. 2001. Bioessays. 23: 327-32. PMID: 11268038
6. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening.  |  Grozinger, CM., et al. 2001. J Biol Chem. 276: 38837-43. PMID: 11483616
7. hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.  |  Vaziri, H., et al. 2001. Cell. 107: 149-59. PMID: 11672523
8. SIRT1 and energy metabolism.  |  Li, X. 2013. Acta Biochim Biophys Sin (Shanghai). 45: 51-60. PMID: 23257294
9. Sirt1 Activity in the Brain: Simultaneous Effects on Energy Homeostasis and Reproduction.  |  D'Angelo, S., et al. 2021. Int J Environ Res Public Health. 18: PMID: 33573212
10. FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1-dependent autophagy.  |  Chen, X., et al. 2022. Br J Pharmacol. 179: 1102-1121. PMID: 34608629
11. Targeting SIRT1-regulated autophagic cell death as a novel therapeutic avenue for cancer prevention.  |  Patra, S., et al. 2023. Drug Discov Today. 28: 103692. PMID: 37379905
12. SIRT1: a novel regulator in colorectal cancer.  |  Dong, W., et al. 2024. Biomed Pharmacother. 178: 117176. PMID: 39059350

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRT1 Antibody (B-10) has 99 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRT1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRT1 Antibody (B-10) Specifications

Alternate Product Name: SIRT1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 448-747

Epitope Target: SIRT1

Epitope Species: human

Product Reactivity/Detection: SIRT1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 99 citations in various scientific publications

Available Conjugates/Forms for SIRT1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin