SIRT1 Antibody (B-10) recommended for detection of SIRT1 of human origin by WB, IP, IF, IHC(P) and ELISA

SIRT1 Antibody (B-10): sc-74504

SIRT1 Antibody (B-10) is rated 4.5 out of 5 by 41.
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Datasheets

    • SIRT1 Antibody (B-10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 448-747 of SIRT1 of human origin
    • SIRT1 Antibody (B-10) is recommended for detection of SIRT1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SIRT1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRT1 (B-10): sc-74504.

Hi, why would one select the B7 over the B10 "version" of this anti-body? They appear very similar? Sorry for the basic question...

Asked by: JSAB434
Thank you for your question. These two SIRT1 monoclonal antibodies are, in fact, very similar. At this time, the main difference between the two clones is that the B-7 clone (sc-74465) is confirmed to be reactive with mouse, rat and human SIRT1, while the B-10 clone (sc-74504) is only confirmed to be reactive with human SIRT1.
Answered by: Technical Support
Date published: 2020-01-30

If using this SIRT1 mouse monoclonal antibody for western blotting, what secondary antibody should I use?

Asked by: Anna99
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 4 out of 5 by from Good Antibody This antibody gave a good and excellent band on Western blot.
Date published: 2020-05-08
Rated 5 out of 5 by from 条带清晰 用来做IP 荧光效果都不错 背景杂带都控制得比较好,特别是用来做荧光 感觉比另外两大抗体公司的产品效果都好,反复使用三次以上都没有问题。
Date published: 2018-04-22
Rated 5 out of 5 by from 条带很干净 一个月前试用了该产品,WB条带很干净,无杂带,特异性高,还没有试过IP和IF,但是不确定沉降效果如何,值得购买
Date published: 2018-03-05
Rated 3 out of 5 by from NOT GOOD I used this antibody in PBMC with 1:200 and the result is not good ,not band
Date published: 2017-10-26
Rated 3 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 4 out of 5 by from good product I applied the free sample, however, I failed to obtain the expeimental result, which mayb e due to the far relativive .
Date published: 2017-09-13
Rated 5 out of 5 by from Western blot Strong signal the SIRT1. We used blocking buffer :5% milk in TBST ,we got clear band at 120kda with no background when diluted the antibody 1:800.
Date published: 2017-09-09
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