SIRT1 Antibody (33.36) recommended for detection of SIRT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SIRT1 Antibody (33.36): sc-135792

SIRT1 Antibody (33.36) is rated 3.5 out of 5 by 2.
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Datasheets

    • SIRT1 Antibody (33.36) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant SIRT1 of human origin
    • recommended for detection of SIRT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See SIRT1 (B-7): sc-74465 for SIRT1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can SIRT1 (33.36): sc-135792 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, SIRT1 (33.36): sc-135792 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from it is suit for IP Great Western Blot data of sirt1 expression in non-transfected and human breast caner cells whole cell lysates
Date published: 2016-11-22
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