POLR3E Antibody (B-11): sc-365703
- POLR3E Antibody (B-11) is a mouse monoclonal IgG1 κ POLR3E antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 53-81 near the N-terminus of POLR3E of human origin
- POLR3E Antibody (B-11) is recommended for detection of POLR3E of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-POLR3E Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for POLR3E Antibody (B-11) for WB applications. These reagents are now offered in bundles with POLR3E Antibody (B-11) (see ordering information below).
POLR3E Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects POLR3E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-POLR3E antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR3E protein, also known as DNA-directed RNA polymerase III subunit RPC5, plays a crucial role in the transcription of small RNA molecules, such as 5S rRNA and transfer RNA, which are essential for protein synthesis and cellular function. Located in the nucleus, POLR3E is one of the seventeen subunits that make up RNA polymerase III, a key enzyme responsible for synthesizing non-coding RNAs that are vital for the proper functioning of the ribosome and the translation process. The gene encoding POLR3E is situated on human chromosome 16, a region associated with various genetic disorders, highlighting the importance of this protein in maintaining cellular health and function. With its ability to facilitate the transcription of critical RNA components, POLR3E (B-11) monoclonal antibody serves as an invaluable tool for researchers studying RNA polymerase activity and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POLR3E Antibody (B-11) References:
- Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex. | Chong, SS., et al. 2001. J Biol Chem. 276: 20727-34. PMID: 11279001
- Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. | Hu, P., et al. 2002. Mol Cell Biol. 22: 8044-55. PMID: 12391170
- [RNA polymerase III transcription apparatus: structure and transcription regulation]. | Nikitina, TV. and Tishchenko, LI. 2005. Mol Biol (Mosk). 39: 179-92. PMID: 15856940
- Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III. | Proshkina, GM., et al. 2006. Nucleic Acids Res. 34: 3615-24. PMID: 16877568
- MicroRNA-directed transcriptional gene silencing in mammalian cells. | Kim, DH., et al. 2008. Proc Natl Acad Sci U S A. 105: 16230-5. PMID: 18852463
- Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells. | Ittmann, M., et al. 1987. Mol Cell Biol. 7: 3386-93. PMID: 3683386
- The BN51 protein is a polymerase (Pol)-specific subunit of RNA Pol III which reveals a link between Pol III transcription and pre-rRNA processing. | Jackson, AJ., et al. 1995. Mol Cell Biol. 15: 94-101. PMID: 7799973
- Cell cycle control of the BN51 cell cycle gene which encodes a subunit of RNA polymerase III. | Ittmann, MM. 1994. Cell Growth Differ. 5: 783-8. PMID: 7947392
- The gene complementing a temperature-sensitive cell cycle mutant of BHK cells is the human homologue of the yeast RPC53 gene, which encodes a subunit of RNA polymerase C (III). | Ittmann, M., et al. 1993. Cell Growth Differ. 4: 503-11. PMID: 8373734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLR3E Antibody (B-11) | sc-365703 | 200 µg/ml | $322.00 | |||
POLR3E Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-529443 | 200 µg Ab; 10 µg BP | $361.00 | |||
POLR3E Antibody (B-11): m-IgGκ BP-HRP Bundle | sc-522336 | 200 µg Ab, 40 µg BP | $361.00 | |||
POLR3E Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-543519 | 200 µg Ab; 20 µg BP | $361.00 | |||
POLR3E Antibody (B-11) AC | sc-365703 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POLR3E Antibody (B-11) HRP | sc-365703 HRP | 200 µg/ml | $322.00 | |||
POLR3E Antibody (B-11) FITC | sc-365703 FITC | 200 µg/ml | $336.00 | |||
POLR3E Antibody (B-11) PE | sc-365703 PE | 200 µg/ml | $349.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 488 | sc-365703 AF488 | 200 µg/ml | $364.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 546 | sc-365703 AF546 | 200 µg/ml | $364.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 594 | sc-365703 AF594 | 200 µg/ml | $364.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 647 | sc-365703 AF647 | 200 µg/ml | $364.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 680 | sc-365703 AF680 | 200 µg/ml | $364.00 | |||
POLR3E Antibody (B-11) Alexa Fluor® 790 | sc-365703 AF790 | 200 µg/ml | $364.00 | |||
POLR3E (B-11) Neutralizing Peptide | sc-365703 P | 100 µg/0.5 ml | $69.00 |