Date published: 2026-1-11

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Pol I/II/III RPB8 Antibody (B8-1): sc-21752

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Datasheets
  • Pol I/II/III RPB8 Antibody (B8-1) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
  • raised against recombinant human RPB8, shared subunit of polymerase I, II and III
  • recommended for detection of RNA polymerase I/II/III subunit RPB8 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pol I/II/III RPB8 Antibody (B8-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Pol I/II/III RPB8 Antibody (B8-1) (see ordering information below).

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Pol I/II/III RPB8 Antibody (B8-1) is a mouse monoclonal IgG1 antibody that detects RPB8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-RPB8 antibody (B8-1) plays a crucial role in the study of RNA polymerases, as RPB8 is a vital subunit shared among all three classes of RNA polymerases—Pol I, Pol II, and Pol III. RPB8 is essential for the assembly and stability of RNA polymerase complexes, which are responsible for synthesizing different types of RNA, including ribosomal RNA and messenger RNA. RPB8 in these complexes is critical for proper transcription of genes, influencing cellular functions and gene expression regulation. Understanding RPB8 interactions and functions can provide insights into transcriptional regulation mechanisms and their implications in various biological processes and diseases. RPB8 monoclonal antibody (B8-1) is therefore an invaluable tool for researchers investigating the intricate roles of RNA polymerases in gene expression and cellular function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Pol I/II/III RPB8 Antibody (B8-1)

sc-21752
200 µg/ml
$322.00

Pol I/II/III RPB8 Antibody (B8-1): m-IgG Fc BP-HRP Bundle

sc-536660
200 µg Ab; 10 µg BP
$361.00

Pol I/II/III RPB8 Antibody (B8-1): m-IgGκ BP-HRP Bundle

sc-533819
200 µg Ab; 40 µg BP
$361.00

Pol I/II/III RPB8 Antibody (B8-1): m-IgG1 BP-HRP Bundle

sc-544744
200 µg Ab; 20 µg BP
$361.00

Can Pol I/II/III RPB8 (B8-1): sc-21752 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Pol I/II/III RPB8 (B8-1): sc-21752 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_1, d_1, h_8CST
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Rated 5 out of 5 by from Worked well in WB in Jurkat whole cell lysateWorked well in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Strong nuclear localization in IHC in formalinStrong nuclear localization in IHC in formalin-fixed human colon tissue. -SCBT QC
Date published: 2013-02-08
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Pol I/II/III RPB8 Antibody (B8-1) is rated 5.0 out of 5 by 2.
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