NPC2 Antibody (H-10) recommended for detection of NPC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NPC2 Antibody (H-10): sc-166321

NPC2 Antibody (H-10) is rated 5.0 out of 5 by 2.
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Datasheets

    • NPC2 Antibody (H-10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 21-145 mapping within an internal region of NPC2 of human origin
    • recommended for detection of NPC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using NPC2 (H-10): sc-166321 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung tissue. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Good Western Blot data of NPC2 expression Good Western Blot data of NPC2 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2014-11-12
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