NPC2 Antibody (D-3): sc-166449

NPC2 Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects NPC2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NPC2 antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NPC2 protein, also known as epididymal secretory protein, is encoded by the gene located at 14q24.3 and plays a crucial role in regulating the lipid composition of sperm membranes during maturation in the epididymis. This function is vital for proper sperm development and fertility, as alterations in lipid composition can affect membrane fluidity and functionality. Mutations in NPC2 gene can lead to Niemann-Pick disease type C2, a severe hereditary condition characterized by defective lysosomal release of cholesterol, resulting in symptoms such as frontal lobe atrophy and other neurological impairments. Notably, absence of HE1, a lysosomal protein, in fibroblasts from NPC2 patients distinguishes this condition from Niemann-Pick type C1, where HE1 is present. NPC2 (D-3) antibody effectively detects and characterizes NPC2 protein, making this reagent an essential tool for research into lipid metabolism and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NPC2 Antibody (D-3) References:

1. Malnutrition and the community--the social origins of deprivation.  |  Longhurst, RW. 1979. Proc Nutr Soc. 38: 11-6. PMID: 111251
2. The integrity of a cholesterol-binding pocket in Niemann-Pick C2 protein is necessary to control lysosome cholesterol levels.  |  Ko, DC., et al. 2003. Proc Natl Acad Sci U S A. 100: 2518-25. PMID: 12591949
3. NPC1 and NPC2 regulate cellular cholesterol homeostasis through generation of low density lipoprotein cholesterol-derived oxysterols.  |  Frolov, A., et al. 2003. J Biol Chem. 278: 25517-25. PMID: 12719428
4. Niemann-Pick disease type C.  |  Vanier, MT. and Millat, G. 2003. Clin Genet. 64: 269-81. PMID: 12974729
5. Genetic evidence for nonredundant functional cooperativity between NPC1 and NPC2 in lipid transport.  |  Sleat, DE., et al. 2004. Proc Natl Acad Sci U S A. 101: 5886-91. PMID: 15071184
6. Secretion of sterols and the NPC2 protein from primary astrocytes.  |  Mutka, AL., et al. 2004. J Biol Chem. 279: 48654-62. PMID: 15355983
7. Pathological cholesterol metabolism fails to modify electrophysiological properties of afflicted neurones in Niemann-Pick disease type C.  |  Deisz, RA., et al. 2005. Neuroscience. 130: 867-73. PMID: 15652985

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NPC2 Antibody (D-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NPC2 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NPC2 Antibody (D-3) Specifications

Alternate Product Name: NPC2

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 21-145

Epitope Target: NPC2

Epitope Species: human

Product Reactivity/Detection: NPC2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NPC2 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin