Nischarin Antibody (G-3): sc-393568

Nischarin Antibody (G-3) is a mouse monoclonal IgG2b antibody that detects Nischarin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nischarin is a novel intracellular protein that plays a crucial role in regulating cell migration by interacting with the cytoplasmic domain of integrin α5/β1 and various members of the PAK family of kinases. Specifically, Nischarin binds to PAK1, inhibiting PAK1′s ability to phosphorylate substrates, which is vital for controlling cellular signaling pathways that govern motility and cytoskeletal dynamics. Nischarin′s localization to membrane ruffles, where Nischarin modulates actin filament organization, underscores Nischarin′s importance in facilitating cell movement. Additionally, Nischarin acts as an antagonist of Rac function, further influencing cell migration and organization. Primarily expressed in the cytoplasm of kidney and brain tissues, Nischarin′s interactions and regulatory functions highlight Nischarin′s potential as a key player in cellular processes related to growth, survival, and motility, making anti-Nischarin antibody (G-3) an essential tool for researchers studying these critical biological functions.

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Nischarin Antibody (G-3) References:

1. Nischarin inhibits Rac induced migration and invasion of epithelial cells by affecting signaling cascades involving PAK.  |  Alahari, SK. 2003. Exp Cell Res. 288: 415-24. PMID: 12915132
2. Inhibition of nischarin expression attenuates rilmenidine-evoked hypotension and phosphorylated extracellular signal-regulated kinase 1/2 production in the rostral ventrolateral medulla of rats.  |  Zhang, J. and Abdel-Rahman, AA. 2008. J Pharmacol Exp Ther. 324: 72-8. PMID: 17940198
3. Imidazoline receptor antisera-selected/Nischarin regulates the effect of agmatine on the development of morphine dependence.  |  Li, F., et al. 2012. Addict Biol. 17: 392-408. PMID: 21967557
4. Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.  |  Kuijl, C., et al. 2013. EMBO J. 32: 713-27. PMID: 23386062
5. Integrin-binding protein nischarin interacts with tumor suppressor liver kinase B1 (LKB1) to regulate cell migration of breast epithelial cells.  |  Jain, P., et al. 2013. J Biol Chem. 288: 15495-509. PMID: 23572524
6. Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.  |  Dong, S., et al. 2017. J Biol Chem. 292: 16833-16846. PMID: 28842496
7. Expression of long noncoding RNA MALAT1 correlates with increased levels of Nischarin and inhibits oncogenic cell functions in breast cancer.  |  Eastlack, SC., et al. 2018. PLoS One. 13: e0198945. PMID: 29912916
8. Nischarin attenuates apoptosis induced by oxidative stress in PC12 cells.  |  Guo, Z., et al. 2019. Exp Ther Med. 17: 663-670. PMID: 30651848
9. Tizanidine (Hydrochloride) Inhibits A549 Lung Cancer Cell Proliferation and Motility Through Regulating Nischarin.  |  Zhao, L., et al. 2020. Onco Targets Ther. 13: 291-298. PMID: 32021275
10. IRAS/Nischarin modulates morphine reward by glutamate receptor activation in the nucleus accumbens of mouse brain.  |  Li, S., et al. 2022. Biomed Pharmacother. 153: 113346. PMID: 36076473

The preferred Nischarin antibody is Nischarin Antibody (F-3): sc-374408.