Nischarin Antibody (F-3): sc-374408

Nischarin Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nischarin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Nischarin antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nischarin plays a crucial role in regulating cell migration, which is vital for processes such as wound healing, immune response, and cancer metastasis. Nischarin (F-3) antibody targets a protein that binds to the cytoplasmic domain of integrin α5/β1 and interacts with members of the p21-activated kinase (PAK) family, particularly PAK1. This interaction inhibits PAK1′s ability to phosphorylate its substrates, thereby affecting downstream signaling pathways that govern cytoskeletal dynamics and cell motility. Nischarin′s localization to membrane ruffles, where Nischarin modulates actin filament organization, underscores its importance in the spatial regulation of cell movement. Primarily expressed in the kidney and brain, Nischarin′s cytoplasmic presence highlights its potential as a key player in cellular responses to environmental cues, making Nischarin a significant target for research in cell biology and pathology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nischarin Antibody (F-3) References:

1. Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration.  |  Alahari, SK., et al. 2000. J Cell Biol. 151: 1141-54. PMID: 11121431
2. IRAS is an anti-apoptotic protein.  |  Dontenwill, M., et al. 2003. Ann N Y Acad Sci. 1009: 400-12. PMID: 15028619
3. Intracellular effect of imidazoline receptor on alpha(2A)-noradrenergic receptor.  |  Chen, MJ., et al. 2003. Ann N Y Acad Sci. 1009: 427-38. PMID: 15028622
4. Relationship between platelet imidazoline receptor-binding peptides and candidate imidazoline-1 receptor, IRAS.  |  Zhu, H., et al. 2003. Ann N Y Acad Sci. 1009: 439-46. PMID: 15028623
5. The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK.  |  Alahari, SK., et al. 2004. EMBO J. 23: 2777-88. PMID: 15229651
6. Human Nischarin/imidazoline receptor antisera-selected protein is targeted to the endosomes by a combined action of a PX domain and a coiled-coil region.  |  Lim, KP. and Hong, W. 2004. J Biol Chem. 279: 54770-82. PMID: 15475348
7. The expression pattern of Nischarin after lipopolysaccharides (LPS)-induced neuroinflammation in rats brain cortex.  |  Wu, X., et al. 2013. Inflamm Res. 62: 929-40. PMID: 24064952
8. Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.  |  Dong, S., et al. 2017. J Biol Chem. 292: 16833-16846. PMID: 28842496
9. Nischarin-siRNA delivered by polyethylenimine-alginate nanoparticles accelerates motor function recovery after spinal cord injury.  |  Ding, YM., et al. 2017. Neural Regen Res. 12: 1687-1694. PMID: 29171434
10. Knockout model reveals the role of Nischarin in mammary gland development, breast tumorigenesis and response to metformin treatment.  |  Dong, S., et al. 2020. Int J Cancer. 146: 2576-2587. PMID: 31525254
11. Nischarin Deletion Reduces Oxidative Metabolism and Overall ATP: A Study Using a Novel NISCHΔ5-6 Knockout Mouse Model.  |  Nguyen, TH., et al. 2022. Int J Mol Sci. 23: PMID: 35163298
12. Preliminary investigation on the mechanism of baicalein regulating the effects of Nischarin on invasion and apoptosis of human breast cancer cells MCF-7 through Wnt3α/β-catenin pathway.  |  He, G., et al. 2024. Int Immunopharmacol. 143: 113262. PMID: 39353394

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nischarin Antibody (F-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Nischarin Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nischarin Antibody (F-3) Specifications

Alternate Product Name: Nischarin

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1212-1504

Epitope Target: Nischarin

Epitope Species: human

Product Reactivity/Detection: Nischarin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Nischarin Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin