Date published: 2025-12-10
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Ku70 Antibody (3C3.11) - Western Blotting - Image 354582
Ku70 Antibody (3C3.11): sc-12729. Western blot analysis of Ku70 expression in MDA-MB-435S (A), C32 (B), K-562 (C), Jurkat (D), MCF7 (E) and SK-MEL-24 (F) whole cell lysates.
Ku70 Antibody (3C3.11): sc-12729. Western blot analysis of Ku70 expression in MDA-MB-435S (A), C32 (B), K-562 (C), Jurkat (D), MCF7 (E) and SK-MEL-24 (F) whole cell lysates.

Ku70 Antibody (3C3.11): sc-12729

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Datasheets
  • Ku70 Antibody (3C3.11) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
  • raised against partially purified extract from HeLa cells
  • recommended for detection of Ku70 of human origin by WB, IP and IF
  • See Ku70 (E-5): sc-17789 for Ku70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ku70 Antibody (3C3.11) for WB applications. These reagents are now offered in bundles with Ku70 Antibody (3C3.11) (see ordering information below).

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SEE ALSO...

Additional Ku Lupus Antigen Antibodies including Ku86

Ku70 Antibody (3C3.11) is a mouse monoclonal IgG1 antibody that detects Ku70 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Ku70 is a critical component of the Ku protein complex, which is essential for DNA repair and maintenance of genomic stability. Ku70 is primarily localized in the nucleus, where anti-Ku70 antibody (3C3.11) can detect its vital role in the non-homologous end joining (NHEJ) pathway, a key mechanism for repairing double-strand breaks in DNA. Nuclear localization of Ku70 ensures ready availability to bind DNA ends and facilitate repair, preventing genomic instability that can lead to cancer and other diseases. Additionally, Ku70 interacts with Ku86 to form a heterodimer exhibiting DNA-binding activity, crucial for recruiting repair factors and activating DNA-dependent protein kinase. Ku70 monoclonal antibody (3C3.11) serves as an invaluable tool for researchers studying DNA repair mechanisms, cellular responses to DNA damage, and disease implications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ku70 Antibody (3C3.11)

sc-12729
200 µg/ml
$316.00

Ku70 Antibody (3C3.11): m-IgG Fc BP-HRP Bundle

sc-536571
200 µg Ab; 10 µg BP
$354.00

Ku70 Antibody (3C3.11): m-IgGκ BP-HRP Bundle

sc-533722
200 µg Ab; 40 µg BP
$354.00

Ku70 Antibody (3C3.11): m-IgG1 BP-HRP Bundle

sc-544684
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with Ku-70 (3C3.11): sc-12729 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great signal strength and specificity inGreat signal strength and specificity in WB with this AB in human Ku-70 transfected 293T and HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear stainingExceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Very strong WB signal obtained using AVery strong WB signal obtained using A-431 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-02
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Ku70 Antibody (3C3.11) is rated 5.0 out of 5 by 4.
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