Date published: 2026-5-5
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Ku70 Antibody (E-5) - Western Blotting - Image 377430
Ku70 Antibody (E-5): sc-17789. Western blot analysis of Ku70 expression in Jurkat (A) and HL-60 (B) nuclear extracts and Daudi whole cell lysate (C).
Ku70 Antibody (E-5): sc-17789. Western blot analysis of Ku70 expression in Jurkat (A) and HL-60 (B) nuclear extracts and Daudi whole cell lysate (C).

Ku70 Antibody (E-5): sc-17789

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Datasheets
  • Ku70 Antibody (E-5) is a mouse monoclonal IgG1 κ Ku70 antibody, cited in 93 publications, provided at 200 µg/ml
  • raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin
  • Ku70 Antibody (E-5) is recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-Ku70 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ku70 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Ku70 Antibody (E-5) (see ordering information below).
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Additional Ku Lupus Antigen Antibodies including Ku86

Ku70 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ku70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Ku70 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ku70, a critical component of the Ku heterodimer alongside Ku86, is primarily located in the nucleus, where Ku70 plays a vital role in DNA repair and maintenance of genomic stability. Ku70 is essential for the non-homologous end joining (NHEJ) pathway, which repairs double-strand breaks in DNA, a common form of damage that can lead to mutations and cancer if left unrepaired. Ku70′s ability to bind to DNA ends and recruit other repair factors underscores Ku70′s importance in cellular responses to DNA damage, making Ku70 a key player in maintaining genome integrity. Ku70 has been implicated in various cellular processes, including transcriptional regulation and cell signaling, further highlighting Ku70′s multifaceted role in cellular homeostasis and response to stress.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ku70 Antibody (E-5) References:

  1. Identification of Ku70 Domain-Specific Interactors Using BioID2.  |  Abbasi, S. and Schild-Poulter, C. 2021. Cells. 10: PMID: 33799447
  2. Ku70 suppresses alternative end joining in G1-arrested progenitor B cells.  |  Liang, Z., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34006647
  3. The Role of Ku70 as a Cytosolic DNA Sensor in Innate Immunity and Beyond.  |  Sui, H., et al. 2021. Front Cell Infect Microbiol. 11: 761983. PMID: 34746031
  4. Integrated analysis reveals FOXA1 and Ku70/Ku80 as targets of ivermectin in prostate cancer.  |  Lv, S., et al. 2022. Cell Death Dis. 13: 754. PMID: 36050295
  5. Signal-induced enhancer activation requires Ku70 to read topoisomerase1-DNA covalent complexes.  |  Tan, Y., et al. 2023. Nat Struct Mol Biol. 30: 148-158. PMID: 36747093
  6. Ubiquitination of Ku70 by Parkin promotes apoptosis of lens epithelial cells.  |  Zhang, W., et al. 2023. FEBS J. 290: 3828-3842. PMID: 37000041
  7. UBE4B interacts with the ITCH E3 ubiquitin ligase to induce Ku70 and c-FLIPL polyubiquitination and enhanced neuroblastoma apoptosis.  |  Le Clorennec, C., et al. 2023. Cell Death Dis. 14: 739. PMID: 37957138
  8. Ku70 senses cytosolic DNA and assembles a tumor-suppressive signalosome.  |  Pandey, A., et al. 2024. Sci Adv. 10: eadh3409. PMID: 38277448
  9. Nonclassical action of Ku70 promotes Treg-suppressive function through a FOXP3-dependent mechanism in lung adenocarcinoma.  |  Huang, Q., et al. 2024. J Clin Invest. 134: PMID: 39446493
  10. Nuclear porcupine mediates XRCC6/Ku70 S-palmitoylation in the DNA damage response.  |  Chen, Y., et al. 2024. Exp Hematol Oncol. 13: 109. PMID: 39497152

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ku70 Antibody (E-5)

sc-17789
200 µg/ml
$322.00

Ku70 Antibody (E-5): m-IgG Fc BP-HRP Bundle

sc-528315
200 µg Ab; 10 µg BP
$361.00

Ku70 Antibody (E-5): m-IgGκ BP-HRP Bundle

sc-520659
200 µg Ab, 40 µg BP
$361.00

Ku70 Antibody (E-5): m-IgG1 BP-HRP Bundle

sc-542862
200 µg Ab; 20 µg BP
$361.00

Ku70 Antibody (E-5) AC

sc-17789 AC
500 µg/ml, 25% agarose
$424.00

Ku70 Antibody (E-5) HRP

sc-17789 HRP
200 µg/ml
$322.00

Ku70 Antibody (E-5) FITC

sc-17789 FITC
200 µg/ml
$336.00

Ku70 Antibody (E-5) PE

sc-17789 PE
200 µg/ml
$349.00

Ku70 Antibody (E-5) Alexa Fluor® 488

sc-17789 AF488
200 µg/ml
$364.00

Ku70 Antibody (E-5) Alexa Fluor® 546

sc-17789 AF546
200 µg/ml
$364.00

Ku70 Antibody (E-5) Alexa Fluor® 594

sc-17789 AF594
200 µg/ml
$364.00

Ku70 Antibody (E-5) Alexa Fluor® 647

sc-17789 AF647
200 µg/ml
$364.00

Ku70 Antibody (E-5) Alexa Fluor® 680

sc-17789 AF680
200 µg/ml
$364.00

Ku70 Antibody (E-5) Alexa Fluor® 790

sc-17789 AF790
200 µg/ml
$364.00

Not sure if I am in the right place but can any of this be used to correct defective genes? An example would be TSC1 on chromosome 9, and TSC2 on chromosome 16. Thanks for a reply from anybody.

Asked by: momof4spoiled1s
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-03-28

Can this antibody be used for ChIP?

Asked by: zire
Thank you for your question. Ku70 Antibody (E-5) is recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in ChIP applications.
Answered by: Tech Support Europe
Date published: 2023-02-28

Can this antibody be used for co-precipitation of Ku70 with other proteins rather than just immunoprecipitation? especially, the interaction between Ku70 and HDAC, or you can recommend a Ku70 that can do HDAC interaction.

Asked by: gongping1125
Thank you for your question. This antibody has not been tested for Co-IP, so we can not say for sure that it will work for that use. It is recommended and warrantied for standard IP use. A good choice for co-IP may be sc-5309 as it does have previous publications for Co-IP use.
Answered by: Tech Service
Date published: 2019-08-29

Can this Ku-70 mouse monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our Ku-70 (E-5): sc-17789 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 7
Date published: 2017-01-19
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Rated 5 out of 5 by from Great antibodyWorks very well by western blot (1:1000 dilution); I saw a clear band at the expected molecular weight
Date published: 2022-02-16
Rated 5 out of 5 by from works greatI used this anti-Ku70 both in WB and IFA, IFA dilute at 1:200, it works very well, there is no non-specific binding , great！
Date published: 2021-12-31
Rated 5 out of 5 by from Good antibody for westernGood specificity and signal strength. Induced upon dsDNA break formation
Date published: 2021-09-14
Rated 5 out of 5 by from Quite clear band for wb of rat protein1:200 diluted for wb of nuclear protein,the band is quite strong
Date published: 2018-05-15
Rated 5 out of 5 by from very strong signalVery robust signal [Western Blots] in mouse spleen tissue.
Date published: 2018-04-19
Rated 5 out of 5 by from Great Immunofluorescence stainingI tried a sample of this antibody on cultured human fibroblasts fixed in Histochoice. The staining was great! I used it at a dilution of 1:200.
Date published: 2017-12-05
Rated 5 out of 5 by from Works for WB/IF/PLAWorks great at 1:1000 for WB, 1:200 for IF. Incredibly pleased with this product!
Date published: 2017-08-30
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
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Ku70 Antibody (E-5) is rated 4.7 out of 5 by 31.
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