IRS-4 Antibody (C-4): sc-393207

IRS-4 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRS-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRS-4 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Insulin receptor substrate (IRS) proteins play a crucial role in mediating signaling pathways initiated by the Insulin receptor, and IRS-4, the most recently identified member of this family, is particularly interesting due to unique expression patterns and potential functions. IRS-4 is primarily expressed in heart and skeletal muscle tissues, where IRS-4 exhibits a limited fiber type-specific expression, highlighting a potential role in muscle metabolism and energy homeostasis. Absence of IRS-4 in knockout mice has been linked to mild defects in growth, reproduction, and glucose regulation, suggesting essential functions in maintaining normal physiology. IRS-4 interacts with phosphatidylinositol 3-kinase (PI3-kinase), which is critical for insulin-stimulated DNA synthesis, and IRS-4 overexpression can enhance PI3-kinase activity, indicating importance in metabolic signaling pathways. IRS-4′s ability to modulate these pathways underscores significance in understanding insulin signaling and implications in metabolic disorders.

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IRS-4 Antibody (C-4) References:

1. Action of insulin receptor substrate-3 (IRS-3) and IRS-4 to stimulate translocation of GLUT4 in rat adipose cells.  |  Zhou, L., et al. 1999. Mol Endocrinol. 13: 505-14. PMID: 10077007
2. IRS-4 mediates protein kinase B signaling during insulin stimulation without promoting antiapoptosis.  |  Uchida, T., et al. 2000. Mol Cell Biol. 20: 126-38. PMID: 10594015
3. IRS-4 mediated mitogenic signalling by insulin and growth hormone in LB cells, a murine T-cell lymphoma devoid of IGF-I receptors.  |  Ursø, B., et al. 2003. Cell Signal. 15: 385-94. PMID: 12618213
4. Role of IRS-4 in PI3-K activation by insulin in HepG2 cells, modulation by Angiotensin II.  |  Villarreal, RS., et al. 2010. Regul Pept. 161: 67-72. PMID: 20079766
5. Association between body mass index and insulin receptor substrate-4 (IRS-4) gene polymorphisms in patients with schizophrenia.  |  Melkersson, K. and Persson, B. 2011. Neuro Endocrinol Lett. 32: 634-40. PMID: 22167131
6. Molecular modulation of the copper and cisplatin transport function of CTR1 and its interaction with IRS-4.  |  Tsai, CY., et al. 2014. Biochem Pharmacol. 90: 379-87. PMID: 24967972
7. Proliferation and migration of hepatoblastoma cells are mediated by IRS-4 via PI3K/Akt pathways.  |  Xia, Z., et al. 2014. Int J Clin Exp Med. 7: 3763-9. PMID: 25419430
8. Actinomycin D Arrests Cell Cycle of Hepatocellular Carcinoma Cell Lines and Induces p53-Dependent Cell Death: A Study of the Molecular Mechanism Involved in the Protective Effect of IRS-4.  |  Guijarro, LG., et al. 2021. Pharmaceuticals (Basel). 14: PMID: 34577545
9. Chronic venous disease patients show increased IRS-4 expression in the great saphenous vein wall.  |  Ortega, MA., et al. 2021. J Int Med Res. 49: 3000605211041275. PMID: 34590920
10. Patients with Invasive Lobular Carcinoma Show a Significant Increase in IRS-4 Expression Compared to Infiltrative Ductal Carcinoma-A Histopathological Study.  |  Ortega, MA., et al. 2022. Medicina (Kaunas). 58: PMID: 35743985

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRS-4 Antibody (C-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-IRS-4 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRS-4 Antibody (C-4) Specifications

Alternate Product Name: IRS-4

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1208-1239

Epitope Target: IRS-4

Epitope Species: human

Product Reactivity/Detection: IRS-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for IRS-4 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IRS-4 antibody is IRS-4 Antibody (C-1): sc-373778.