DHFR Antibody (C-4): sc-393154
- DHFR Antibody (C-4) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 77-106 within an internal region of DHFR of human origin
- recommended for detection of DHFR and DHFRL1 of human origin and DHFR of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- See DHFR (A-9): sc-377091 for DHFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DHFR Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with DHFR Antibody (C-4) (see ordering information below).
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SEE ALSO...
DHFR Antibody (C-4) is a mouse monoclonal IgG2b light chain antibody that detects dihydrofolate reductase (DHFR) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DHFR plays a pivotal role in cellular metabolism by catalyzing the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, which is essential for the synthesis of purines, pyrimidines, and certain amino acids. This enzymatic activity is crucial for DNA synthesis; thus, inhibition of DHFR can lead to cell cycle arrest and apoptosis, making DHFR a significant target in cancer therapy. Notably, the expression of methotrexate-resistant variants of DHFR in normal hematopoietic cells presents a promising strategy to enhance the therapeutic window of methotrexate by reducing toxicity to healthy tissues while allowing for higher doses to be administered. Anti-DHFR antibody (C-4) serves as an invaluable tool for researchers studying cellular processes and therapeutic interventions, given DHFR′s interactions with various substrates and inhibitors in both normal physiology and disease states.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHFR Antibody (C-4) | sc-393154 | 200 µg/ml | $316.00 | |||
DHFR Antibody (C-4): m-IgG Fc BP-HRP Bundle | sc-538156 | 200 µg Ab; 10 µg BP | $354.00 | |||
DHFR Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-535716 | 200 µg Ab; 40 µg BP | $354.00 | |||
DHFR Antibody (C-4): m-IgG2b BP-HRP Bundle | sc-549922 | 200 µg Ab; 10 µg BP | $354.00 | |||
DHFR (C-4) Neutralizing Peptide | sc-393154 P | 100 µg/0.5 ml | $68.00 |